黄金香柳组织培养与快速繁殖

Rapid Propagation of Melaleuca bracteata F.Muell.via Tissue Culture

文章报道黄金香柳的组织培养和快速繁殖试验。结果表明：以黄金香柳嫩茎作外植体进行培养,不定芽诱导率高,经连续3次培养不定芽增殖倍数可达7.9～10.7。适宜黄金香柳丛芽快速增殖的培养基配方为MS＋6-BA 0.5 mg/L＋NAA 0.01 mg/L＋蔗糖30 g/L,一次继代培养时,平均每瓶无菌材料可提供45.5个枝芽（≥1 cm）用于转接生根。诱导植株生根的最佳培养基配方为MS＋IBA 1.0～1.5 mg/L＋蔗糖15 g/L,适宜的培养时间为21～25 d,生根率可高达96%以上。移植的试管植株90%以上存活。

This paper deals with the preliminary, results on tissue culture and rapid propagation of Melaleuca bracteata F. Muell.. After 3 subcultures, a large number of adventitious buds could be induced from shoot explants on MS and DCR media supplemented with 6-BA 0.5 - 2.0 mg/L and NAA 0,0.1 mg/L, on which increased the multiple buds nearly tenfold. Nevertheless, the optimum propagation medium for subculture selected from the experiments was MS containing 6-BA 0.5 mg/L and NAA 0. 01 mg/L, under this procedure be able to obtain 45.5 shoots for rooting culture from one culture bottle in a period of subculture averagely. The trial also identified that the optimum medium for root induction was MS supplemented with IBA 1.0 - 1.5 mg/L and 96% of rooting percentage was obtained. The plantlet survival rate could exceed 90% provided that the nursery condition was acceptable.