创伤合并海水浸泡后愈合过程的病理学观察

Pathological Study on the Wound Healing of the Seawater Immersion Wound

目的观察创伤合并海水浸泡后对伤口愈合时间的影响及愈合过程中的病理学改变。方法以大鼠为实验动物,建立背部双侧圆形创伤模型,创后随机分为对照组和实验组,每组50只,实验组创后置海水中浸泡30min。观察2组创面的愈合生长状况。观察伤口局部愈合过程中的病理学改变,应用免疫组化方法观察愈合过程中伤口修复细胞增殖指数及微血管密度变化。结果对照组平均愈合时间为(12.0±1.0)d,而实验组的平均愈合时间为(14.3±0.8)d;两组在肉芽组织形成时间上以及修复细胞增殖能力上存在差异。结论创伤合并海水浸泡可导致伤口愈合时间延迟,加重创伤局部的炎症反应、延缓肉芽组织形成时间。

Objective To observe and pathological study the effect of seawater immersion on the wound healing course.Methods The wound models on skin of rats were made and randomly divided into two groups.One was control group（n=50）;the other was seawater immersion group（experimental group,n=50）,in which group the animals were immersed into the seawater about 30 minutes after making the wound.The wound areas were recorded,the growth of wound granulation was observed,the proliferation index of the fibroblast cells and microvessel density of the wound was studied by immunohistochemistry.Results The mean healing time of the wounds in control group was（12.0±1.0）d;（14.3±0.8）d when treated by seawater immersion.The formation time of the granulation tissue and the proliferation ability were different in two groups.Conclusions Seawater immersion can enhance inflammatory response of the wound,slow down the growth of the granulation tissue,and delay the wound healing.