肌苷酸合成酶系3个基因对白耳鸡胸肌肌苷酸含量的遗传效应

Analysis of the Genetic Effects of Three Genes Involved in de novo Purine Biosynthesis on IMP Content in Baier Chicken

GPAT、AIRC和PURH基因作为肌苷酸合成过程中的酶系基因,它们对鸡肌肉肌苷酸含量均具有显著的效应。为了进一步验证这3个基因对肌苷酸含量的遗传效应,本研究以白耳鸡为试验材料,采用PCR-SSCP和测序相结合的方法,分析了这3个基因的单基因以及合并基因型对鸡胸肌肌苷酸含量的影响。结果表明:GPAT、AIRC和PURH基因均对白耳鸡胸肌肌苷酸含量的差异产生影响,均与鸡肌肉IMP含量存在显著关联(P<0.05),这3个位点可作为影响鸡肌肉肌苷酸含量的分子标记;合并基因型的遗传效应要高于最优秀的单个基因型效应。因此,可以利用合并基因型对鸡的肉质风味性状进行标记辅助选择。

GPAT,AIRC and PURH genes are involved in the de novo purine biosynthesis, they all have significant effect on muscle IMP content. This study was designed to investigate the effect of GPAT,AIRC and PURH genes on muscle IMP content in Baler chickens, single genotype effects as well as the combined genotype effects were all studied. PCR-SSCP and DNA sequencing were applied to detect SNPs in three genes. The results showed that GPAT,AIRC and PURH genes bad significant effect on the muscle IMP content in Baler chickens （P〈0.05）, they are candidate loci or linked to major genes that affect muscle IMP content; The combined genotype effects were higher than the single genotype effects. It was concluded that combined genotypes are superior to single genotypes as potential molecular markers for meat quality traits in chicken.