填饲后朗德鹅肝脏和小肠组织中MTP基因表达研究

Study on Gene Expression of MTP in the Liver and Small Intestine Tisssues in Overfed Landes Goose

为了探讨填饲对MTP基因表达的影响,本研究采用RT-PCR法检测了填饲后MTP基因在朗德鹅肝脏和小肠组织中的表达情况,并对6种体组成参数和甘油三酯等4种血清生化指标进行了检测。结果表明:填饲后,各体组成参数和血清生化指标的水平都显著升高;MTP基因在朗德鹅的肝脏和小肠组织中都有表达;填饲后肝脏组织中MTP基因的表达无显著性变化(P>0.05),而小肠组织中的MTP基因的表达水平却显著升高(P<0.05)。结论:填饲引起MTP基因在小肠和肝脏中的变化差异可能与肝细胞中可利用脂类物质的水平、其它调控因子及填饲的高脂食物有关。

In order to study the effect of overfeeding on gene expression of MTP, the gene expression level of MTP mRNA in intestine and liver tissues of Landes geese was detected by RT-PCR, which were collected from the control and overfeeding treatment groups. The relevant 6 body composition parameters and 4 serum parameters were studied. The results showed that the detected parameters increased significantly. The MTP mRNA was expressed in intestine and liver tissues. Compared the control group, after overfeeding, the level of MTP gene mRNA had no significant change in liver （P〉0.05）, and the level of MTP gene mRNA increased significantly （P〈0.05） in small intestine. In conclusion, the change difference of the MTP mRNA level induced by overfeeding might be related to the lipids level which could be used in hepatocytes, other regulating factors in vivo and the high fat diets.