Asia1型口蹄疫病毒VP2蛋白B细胞线性表位的鉴定

Identification of Linear B-cell Epitope of Structural Protein VP2 of Foot-and-Mouth Disease Virus Serotype Asia1

为了对Asia1型口蹄疫病毒VP2蛋白进行抗原表位作图,设计了7个相互重叠15个氨基酸、覆盖整个VP2蛋白的重叠短肽,经克隆并在大肠杆菌中实现了融合表达。用Asia1型口蹄疫感染血清对7个融合蛋白进行免疫印迹分析,结果初步鉴定出VP2蛋白B细胞线性抗原表位位于氨基末端的第1-44氨基酸区域,并且证实O型、A型、C型口蹄疫标准血清和感染SAT2型口蹄疫康复期的牛血清也能识别融合蛋白F1(1-44 aa)。在此基础上,针对F1(1-44 aa)短肽,设计了6个相互重叠5个氨基酸的短肽片段,进一步鉴定出了VP2蛋白B细胞线性表位位于氨基末端的第6-15氨基酸区域。

In order to identify the linear B cell epitope of structural protein VP2 of foot-and-mouth disease virus（FMDV） serotype Asia1, the VP2 protein was dissected into seven overlapping fragments, and expressed in Escherichia coli, respectively. Western blot analysis of the seven fused proteins was carried out with sera from FMD infected cattle. The results showed that the linear B-cell epitope of VP2 protein is located in the 1-44 aa of N-terminal region. We also found that serotype O, serotype A, serotype C FMD standard serum and infected serotype SAT2 FMD reha bilitation period bovine serum could also identify the epitope fusion protein. Based on these, the short peptide protein F1 was dissected into six overlapping fragments further. Finally, we identified that the linear B cell epitope on VP2 is located in the 6-15 amino acid of N-terminal region.