A-FABP和AMPD1基因SNP位点对北京油鸡部分肉质性状的组合效应分析

The Effect of Genotype Combinations between the Single Nucleotide Polymorphisms of A-FABP and AMPD1 Genes on the Partial Meat Quality Traits in Beijing-You Chicken

本研究旨在分析鸡脂肪型脂肪酸结合蛋白(A-FABP)和腺苷一磷酸脱氨酶(AMPD1)基因的SNP位点对鸡肉肌内脂肪(IMF)和肌苷酸(IMP)含量的组合效应。试验以北京油鸡为材料,采用PCR-SSCP技术对A-FABP基因外显子1第51位碱基(P4位点)的C→T突变和AMPD1基因序列第129位(PB位点)的A→G突变进行单基因和多基因组合效应分析。结果表明,P4位点的突变纯合BB基因型和PB位点的突变纯合NN基因型分别是多态位点上IMF和IMP含量的优势基因型;组合效应分析结果表明,在9种组合中,BBNN和BBMN组合基因型个体的组合效应显著,其中BBMN基因型个体的IMF含量、腹脂率(AFP)和平均脂带宽(WFS)比组合前P4位点BB基因型和PB位点MN基因型个体的均值分别增加了7.80%、50.74%和4.92%,组合效应显著(P<0.05),IMP含量比组合前增加了2.53%;2个基因位点间不存在显著的互作效应(P>0.05)。因此,可将BB与NN或MN基因型聚合,进行分子标记聚合育种,以辅助选择提高鸡肉IMP和IMF含量。

This study was conducted to analyze the effect of genotype combinations between the single nucleotide polymorphisms （SNPs） of adipocyte fatty acid binding protein （A-FABP） gene and adenosine 5＇-monophosphate deaminase 1 （AMPD1） gene on the partial meat quality traits in Beijing-You chickens. 160 purebred geijing-You male chickens were used. The SNPs of the base mutation C→T at position 51 （P4 site） for A-FABP gene and mutation A→G at position 129 （PB site） for AMPD1 gene were detected by PCR-SSCP. The results showed that BB genotype for A- FABP gene and NN genotype for AMPD1 gene were acted on IMF and IMP contents respectively as the superior genotypes for their own polymorphic sites. Among nine different genotype combinations, BBNN and BBMN genotypes were the dominant combination. The content of IMF and percentage of abdominal fat （AFP） and width of fat strip （WFS） of individuals with BBMN genotype were 7.80%, 50.74% and 4.92% significant higher than the average value of individual BB and MN genotype respectively（P〈0.05）, and 2.53% higher on IMP. However, there is no interacted effect between two sites （P〉0.05）. In conclusion, IMF and IMP contents could be improved by applying marker-assisted selection （MAS） of combining P4 site for A-FABP gene and PB site for AMPD1 gene in Beijing-You chicken.