门静脉高压症患者脾切除后外周血B7-1/CD28表达的变化

Expression changes of B7-1/CD28 in peripheral blood lymphocytes of patients with portal hypertension after splenectomy

目的探讨B7-1/CD28对门静脉高压症(portal hypertension,PHT)患者免疫功能的影响和作用。方法采用流式细胞分析技术对30例门静脉高压症患者进行研究,检测门静脉高压症患者行脾切除前后外周血淋巴细胞中以及脾脏中B7-1/CD28的表达情况,分析其与患者淋巴细胞亚群以及免疫功能的关系。对患者外周血B7-1/CD28和淋巴细胞进行检测。利用免疫组化方法检测B7-1/CD28在脾脏中的表达,与10例外伤性脾破裂行脾切除之脾脏组织进行比较。结果门静脉高压症患者外周血中淋巴细胞亚群和B7-1/CD28表达较低,脾切除后短期内升高。其脾脏中表达的B7-1/CD28较正常人群高。结论门静脉高压症患者全脾切除后外周血B7-1/CD28通路被激活,患者免疫功能得到部分恢复。

Objective To study the effect of B7-1/CD28 on immunological function in patients of portal hypertension after appendectomy. Methods The data of thirty patients with portal hypertension and ten trau- matic patients were analyzed. The changes of CD20, CD4, CD8, CD4/CD8 and B7-1/CD28 in the peripheral blood were detected by FCM and B7-1/CD28 in spleen was detected with immunohistoehemisty. The relationships between them were investigated. Results The expression of CD20, CD4, CD8, CD4/CD8, B7-1/CD28 in the peripheral blood lymphocytes decreased. They recovered in short time after appendectomy. Conclusion The pathway of B7-1/CD28 is activated after splenectomy and the immunological function of PHT patients recovers partly.