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IL-1β及IL-1ra对结肠癌细胞表达uPA的影响 被引量：5

Effects of interleukin-1β and interleukin-1 receptor antagonist on the expression of urokinase-type plasminogen activator in colon carcinoma cells
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摘要 目的研究白细胞介素(IL)-1β和白细胞介素受体拮抗剂(IL-1ra)对结肠癌细胞表达uPA的影响,探讨IL-1β对结肠癌细胞迁移能力的影响。方法体外培养人结肠癌细胞,加入IL-1β和IL-1ra,应用半定量逆转录-聚合酶链反应方法,比较不同处理组结肠癌细胞中uPAmRNA的表达。结果IL-1β可促进结肠癌细胞的生长增殖,上调uPAmRNA的表达,呈一定的剂量效应关系;在时间系列组中,可检测到uPA mRNA的表达,以24 h表达最高。IL-1ra可以抑制IL-1β对uPA mRNA表达的上调作用。结论IL-1β促进结肠癌细胞生长增殖,刺激结肠癌细胞uPA高表达,从而促进结肠癌的迁移,IL-1ra可抑制IL-1β的作用。 Objective To study the effects of interleukin-1β（IL-1β） and interleukin-1 receptor antagonist（IL-1ra） on the expression of urokinase-type plasminogen activator（uPA） in colon carcinoma cells,and to explore the effect of IL-1β on the migration of colon carcinoma cells. Methods Colon carcinoma cells were cultured in vitro and treated with IL-1β and IL-1ra,the expression of uPA mRNA was examined by semi-quantitative RT-PCR. Results IL-1β could promote the proliferation of colon carcinoma cells and up-regulate the expression of uPA mRNA in a dose-dependent way.In the time series,the expression of uPA mRNA at 24 hours was the highest.The effect was inhibited by IL-1ra. Conclusion IL-1β promotes the proliferation and induces the expression of uPA in colon carcinoma cells.IL-1β can stimulate the migration of colon carcinoma cells.IL-1ra can block the action of IL-1β.
作者 张佳 罗和生 钟清连
机构地区 武汉大学人民医院消化科
出处 《胃肠病学和肝病学杂志》 CAS 2009年第10期942-944,共3页 Chinese Journal of Gastroenterology and Hepatology
关键词 IL-1 βIL-1ra UPA 结肠癌细胞 RT-PCR IL-1β IL-1ra uPA Colon carcinoma cells RT-PCR
分类号 R735.35 [医药卫生—肿瘤]
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参考文献12

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同被引文献40

  • 1宋相伟,杨婉身,周晓巍,杨志新,黄培堂.白细胞介素-1信号转导调控研究进展[J].细胞与分子免疫学杂志,2005,21(B03):3-5. 被引量：9
  • 2陈江辉,李登清,陈子华.结直肠癌患者IL-6基因启动子-174G→C多态性研究[J].中国普通外科杂志,2007,16(4):338-340. 被引量：3
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胃肠病学和肝病学杂志
2009年 第10期

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