IαIP、IL-8在新生儿细菌感染早期诊断中的意义

Significance of serum IαIP and IL-8 for early diagnosis in neonates with bacterial infections

目的探讨血清inter-α抑制蛋白(IαIP)、白细胞介素8(IL-8)在新生儿细菌感染早期诊断中的意义。方法新生儿分为感染(A)组、非感染(B)组和正常对照(C)组。研究对象,在起病12 h内(T1)、24 h(T2)及恢复期(T3)采血检测IαIP及IL-8。比较单独及联合检测IαIP、IL-8早期诊断新生儿细菌感染的灵敏度、特异度、阳性和阴性预告值。结果与B、C组比较,A组在T1、T2时的IαIP明显降低,IL-8明显升高。当分别以灰度值16.5和血清值70 pg/ml作为IαIP、IL-8的最佳截断值时,IL-8有最高特异度(95.45%),但灵敏度不高;IαIP有较高的灵敏度,在起病12 h内和24 h分别为95.45%和81.82%。联合检测IαIP和IL-8灵敏度均达95.45%,而特异度分别为77.27%和81.82%。结论联合检测IαIP和IL-8可作为新生儿细菌感染早期诊断的可靠指标。

Objective To explore the clinical significance of inter-alpha inhibitor protein （IαIP） and interleukin-8 （IL-8） for early diagnosis of neonatal bacterial infections. Methods The newborns were divided into infection group（A）,non-infection group（B） and normal control group（C）. IαIP and IL-8 were measured within 12 h after the onset of disease（T1） ,at 24 h（T2） and during recovery period （T3）. The sensitivity,specificity, positive predict value （PPV） and negative predict value （NPV） of two markers were calculated and compared. Results Compared to groups of B and C,IαIP was lower and IL-8 higher in group A at TI and T2 （P〈0. 05）. Taking the gray value 16.5 of IαIP and the serum concentration 70 pg/ml of IL-8 respectively as the optimal cutoff value, examination of IL-8 had a highest specificity of 95. 45% but relatively low sensitivity. Examination of IαIP had a high sensitivity of 95.45% at T1 and 81. 82% at T2. Combined examination of IαIP and IL-8 had the highest sensitivity of 95.45% at T1 and T2 and the specificities were 77. 27% and 81.82% at T1 and T2. Conclusion Combined examination of IαIP and IL-8 can be taken as the markers for early diagnosing neonatal bacterial infections.