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脂肪分化相关蛋白促进RAW264.7细胞内酰基辅酶A∶胆固醇酰基转移酶1高表达 被引量：2

Adipophilin Facilitate Acyl-Coenzyme A∶Cholesterol Acyltransferase 1 Expression in RAW264.7 Cells
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摘要 目的观察高表达脂肪分化相关蛋白细胞内酰基辅酶A∶胆固醇酰基转移酶1表达的变化,阐明脂肪分化相关蛋白促进细胞内脂质蓄积的机制。方法构建的pQCXIP-HA-Adi逆转录病毒载体转染PA317包装细胞,获得pQCXIP-HA-Adi逆转录病毒。用病毒感染RAW264.7细胞,Puromycin筛选后获得稳定高表达脂肪分化相关蛋白的RAW264.7细胞株。应用逆转录聚合酶链反应和免疫印迹法检测经感染后细胞内脂肪分化相关蛋白和酰基辅酶A∶胆固醇酰基转移酶1的表达。并应用阿托伐他汀处理高表达脂肪分化相关蛋白的RAW264.7细胞,观察酰基辅酶A∶胆固醇酰基转移酶1表达的改变。结果用pQCXIP-HA-Adi逆转录病毒感染RAW264.7细胞后,脂肪分化相关蛋白和酰基辅酶A∶胆固醇酰基转移酶1 mRNA和蛋白表达明显升高,而对照组表达无明显变化。加入阿托伐他汀后,即在去除底物对酰基辅酶A∶胆固醇酰基转移酶1表达影响的情况下,高表达脂肪分化相关蛋白细胞内酰基辅酶A∶胆固醇酰基转移酶1表达仍升高。结论高表达脂肪分化相关蛋白可明显上调RAW264.7细胞酰基辅酶A∶胆固醇酰基转移酶1的表达。 Aim To explore whether adipophilin affects the expression of acyl-coenzyme A∶cholesterol acyltransferase 1（ACAT1） and illuminate the lipid-accumulation mechanism mediated by adipophilin. Methods The recombinant retroviral vector pQCXIP-HA-Adi was constructed and transfected into packaging cell PA317 mediated by SofastTM,which can induce retrovirus release.Then this retrovirus were used to infect RAW264.7 cell and got RAW264.7 cell line which highly expressed adipophilin.The mRNA level of adipohilin and ACAT...
作者 乔运成 黄谙非 田国平 刘晓辉 袁中华
机构地区 河南省平舆县人民医院心血管内科 深圳市西丽人民医院病理科 南华大学附属第二医院心血管内科 南华大学心血管病研究所动脉硬化学湖南省重点实验室
出处 《中国动脉硬化杂志》 CAS CSCD 北大核心 2009年第8期643-647,共5页 Chinese Journal of Arteriosclerosis
基金 教育部留学回国人员科研启动基金(教外司留〔2008〕890号)
关键词 脂肪分化相关蛋白 酰基辅酶A∶胆固醇酰基转移酶1 RAW264.7细胞 动脉粥样硬化 Adipophilin Acyl-Coenzyme A∶Cholesterol Acyltransferase 1 RAW264.7 Cells Atherosclerosis
分类号 R363 [医药卫生—病理学]
  • 相关文献

参考文献15

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二级参考文献6

共引文献14

同被引文献36

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引证文献2

二级引证文献2

中国动脉硬化杂志
2009年 第8期

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