神经生长因子及受体在非黑素性皮肤癌中的表达

Expression of Nerve Growth Factor and its Receptor in Non-Melanoma Skin Cancer

目的:探讨神经生长因子(NGF)及受体P75在非黑素性皮肤癌中的表达情况。方法:用原位杂交的方法对15例鳞状细胞癌(SCC)、12例基底细胞癌(BCC)、13例正常上皮(NS)中NGF及受体P75 mRNA进行了检测。结果:与正常皮肤组织比较,NGF mRNA在基底细胞癌组织中的表达明显增高(t=30.06,P<0.01),在鳞状细胞癌组织中的表达明显下降(t=19.62,P<0.01),鳞状细胞癌组织与基底细胞癌组织相比差异有统计学意义(t=57.36,P<0.01)。P75mRNA在皮肤正常组、基底细胞癌组、鳞状细胞癌组中的表达呈逐渐降低趋势,组与组之间两两比较差异均有统计学意义:鳞状细胞癌组与正常皮肤组(t=42.69,P<0.01),基底细胞癌组与正常皮肤组(t=30.37,P<0.05),鳞状细胞癌组与基底细胞癌组(t=18.49,P<0.05)。结论:NGF及受体P75可能在非黑素性皮肤癌发生及浸润中起一定作用。

Objective：To investigate expression of nerve growth factor （NGF） and its receptor （ P75 ） in non - melanoma skin cancer. Methods： To investigate expression of nerve growth factor （NGF） and its receptor （P75） in non - melanoma skin cancer. The expression of NGF and p75mRNA was detected by in situ hydridization in paraffin sections of 30 skin samples eomparising 15 cases of squamous cell carcinoma（ SCC）, 12 basal cell carcinoma, 13 normal skin（NS）. Resuits：As compared to normal skin, expression of NGFmRNA was significantly enhanced in BCC （t =30. 06 ,P 〈0. 01 ） and significantly decreased in SCC （t = 19.62,P 〈0. 01 ）. NGFmRNA expression also significantly differed between BCC and SCC （t = 57.36,P 〈 0. 01 ）. P75mRNA expression was decreased in NS, BCC and SCC. Comparisons between the following groups revealed significant differences： NS and SCC（t =42.69 ,P 〈0.01 ）, NS and BCC（t =30. 37 ,P 〈0. 05）, BCC and SCC （ t = 18.49, P 〈 0. 05 ）. Conclusion： NGF and its receptor P75 play an important role in tumor occurrence and invasion in non melanoma skin cancer.