大豆异黄酮抗肝纤维化的实验研究

Empirical study of inhibitive effect of isoflavone on hepatic fibrosis

目的观察大豆异黄酮是否具有抗肝纤维化的作用，探讨大豆异黄酮抗肝纤维化的可能机制。方法清洁级SD雄性大鼠随机分成4个组，模型组用硫代乙酰胺建立肝纤维化模型，治疗组建立肝纤维化模型的同时给予大豆异黄酮干预。生理盐水对照组相同途径给予生理盐水，大豆异黄酮对照组相同途径给予生理盐水和大豆异黄酮。检测血清CIV、HA水平和观察肝脏病理，并作统计学分析。结果生理盐水对照组、模型组和治疗组的血清cⅣ水平分别为（11．17±1．89）ng／ml、（70．66±6．06）ng／ml和（50．10±8．86）ng／ml（P〈0．05），治疗组的血清HA水平明显低于模型组（P〈0．05），但高于生理盐水对照组（P〈0．05）。生理盐水对照组和大豆异黄酮对照组的血清CⅣ、HA水平不存在统计学差异（P〉0．05）。生理盐水对照组、模型组和治疗组的肝纤维化记分分别为0、11．64±3．72、8．83±3．83（P〈0．05）。结论大豆异黄酮具有抑制慢性肝损伤所致的肝纤维化形成的作用，其机制可能为通过减少细胞外基质的形成。

Objective To observe if isoflavone has the inhibitive effect on hepatic fibrosis and explore its possible mechanism. Methods Cline male SD rats were divided into four groups. The rats in the model group were given thioacetamide for establishing the model of hepatic fibrosis, those in the treatment group was given isoflavone to establish the model of hepatic fibrosis, those in the normal saline control group was given normal saline and the animals in the isoflavone control group was given normal saline and isoflavone by the same way. The levels of CIV and HA in serum were determined and pathological changes of liver observed. Results The serum level of CIV in the normal saline control group, the model group and the treatment group was （11.17±1.89） ng/ml, （70.66±6.06） ng/ ml and （50.10±8, 86） ng/ml, respectively （P〈0.05）. The serum level of HA was significantly lower in the treatment group than in the model group （P〈0. 05） but higher than in the normal saline control group （P〈0.05）. There was no statistical difference in serum levels of CIV and HA between the normal saline control group and the isoflavone control group （P〉0. 05）. The score of hepatic fibrosis of rats in the normal saline control group, model group and the treatment group was 0, 11.64±3.72 and 8. 83±3.83, respectively （P〈0.05）. Conelusion Isoflavone has the inhibitive effect on hepatic fibrosis and its possible mechanism might be to inhibit the forming of extracellular matrix.