摘要
目的探讨B7-H4分子在小鼠树突状细胞分化发育过程中的表达及其在T细胞活化中的作用。方法采用GM-CSF和IL4联合方案体外诱导小鼠髓系树突状细胞（DCs）；采用流式细胞术检测未成熟DCs、成熟DCs以及IL-10诱导的DCs表面B7-H4分子的表达；采用。H—TdR掺入试验和抗B7-H4单抗（McAb）阻断试验分析DCs表达的B7-H4分子对T淋巴细胞的共刺激效应。结果经GM-CSF和IL-4联合方案体外诱导的未成熟DCs较高水平表达B7-H4，在IL-10作用下B7-H4表达进一步上调，经TNF-α刺激成熟后，B7-H4的表达显著下调，不同功能状态下DCs表面B7-H4分子均可抑制T细胞的增殖，但以未成熟DCs、IL-10诱导的DCs表面B7．H4分子的抑制作用更为显著。结论不同功能状态下的DCs均有B7-H4分子的表达，处于抑制状态下的DCs通过高表达B7-H4介导免疫不应答效应。
Objective To study the role of B7-H4 on the surface of murine DCs in mediating T cell activation. Methods Mouse myeloid dendritic ceils （DCs） were generated from bone marrow in vitro using GM-CSF and IL-4, and further stimulation with TNF-α or IL-10 for 48 h, respectively. Expressions of B7-H4 on DCs were analyzed by FCM. 3 H-thymidine incorporation test was used to detect the T cell proliferation stimulated by DCs with or without blocking B7-H4 by MeAb, respectively. Results 5-6 d DCs in our system can be regarded as imDCs. B7-H4 was expressed moderately on imDCs, and could be markedly upregulated by IL-10, and be markedly down-regulated by TNF-α. 3 H-thymidine incorporation test showed that blockade of B7-H4 on imDCs and IL-10-treated DCs resulted in enhancement of T cell proliferation significantly. When T cells encountered with mDCs, B7-H4 blockade could not markedly enhance the T cells proliferation as seen in inhibitory DCs. Conclusion Expression of B7-H4 on inhibitory DCs might contribute to its immunoinhibitory effect.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2009年第10期903-906,共4页
Chinese Journal of Microbiology and Immunology
基金
国家重点基础研究发展规划（973计划）资助项目（2007CB512402）
国家自然科学基金资助项目（30700728）
苏州大学附属第一医院科研项目（YT0712）
