斑点叉尾鮰病毒宿主研究

A study on the host range of the Channel catfish virus infection

为了解斑点叉尾鮰病毒(CCV)感染宿主范围和CCV病暴发的水温条件。本研究将斑点叉尾鮰、鳙鱼、加州鲈、鲫鱼、南方大口鲇、鲤鱼、胭脂鱼、岩原鲤、四川白甲、草鱼鱼苗各250尾,体质量约为25g,随机分成102组,每组20尾,分别通过浸泡感染、胸腔感染和胸腔感染暴发温度实验,实验期14d。结果表明,加州鲈和南方大口鲇对CCV非常易感,25℃斑点叉尾鮰和南方大口鲇死亡50%,加州鲈40%死亡;28℃斑点叉尾鮰和南方大口鲇全部死亡,加州鲈75%死亡;鲤鱼也会感染但不死亡,实验的其它鱼类没有组织病理学改变和临床症状出现。研究结果表明CCV对加州鲈和南方大口鲇敏感,暴发温度为25℃。

The host range and the outbreak water temperature of Channel catfish virus （CCV） were investigated through experimental CCV infection. Each of 250 Ictalurus punctatus, Aristichthys nobilis, Micropterus salmoides, Carassius carassius, Silurus meriaionalis, Cyprinus carpio, Myxocyprinus asinaticus, Procypris rabaudi, Varicorhinus angustistomatus and Ctenopharyngodon idellus, respectively, weighting an average of 25 ±0.25 g, were divided into 102 groups with 20 in each group. The test groups were infected with CCV by immersion or injection and monitored for 14 days. The results showed that （1） Micropterus salmoides and Silurus meriaionalis were very susceptibility to CCV infection, about 50 % mortality observed in Ictalurus punctatus and Silurus meriaionalis. The Micropterus salmoides had 40 % mortality at temperature above 25 ℃. （2） 100 % motality in Ictalurus punctatus and Silurus meriaionalis and 75 % death in Micropterus salmoides occurred at temperature 28 ℃. （3） Cyprinus carpio were infected but had no mortality. No deaths and clinical or histopathological signs were found in six other species exposed by immersion or injection. These experiments confirmed that Micropterus salmoides, Silurus meriaionalis is very susceptibility to CCV infection. The outbreaks of CCV disease only occurred at temperature above 25 ℃.