大花萱草组培快繁体系的研究

Research on Tissue Culture Regeneration of Hemerocallis middendorfii

以大花萱草的6个品种为试材,主要研究了其组织培养中的激素配比、外植体类型、基因型以及不定芽的生根条件。结果表明:适合于大花萱草紫蝶的最佳愈伤组织及不定芽诱导培养基为MS+1mg·L-16-BA+0.1mg·L-1NAA,出愈率、分化率和平均出芽数分别为63.33%、91.11%和5.86;但是,上述指标在基因型间表现出差异;花茎是诱导不定芽的最佳外植体;1/2MS+0.2mg·L-1NAA是紫蝶较适宜的生根培养基,生根率81.11%,平均生根数6.08;本实验建立了3个品种的再生体系,平均出芽数均在4个以上,其中"金娃娃"最高,是6.88。

Using Hemerocallis middendorfii＇ s 6 varieties as experimental materials, we the investigated effects of hormone combination, types of explants, genotype and culture media on rooting of adventitious shoot. The results showed that, the best medium of Little bumble bee for callus and adventitious shoot differentiation was MS ＋ 1 mg·L^-1 6-BA ＋ 0.1 mg ·L^-1 NAA, its callus induction rate, differentiation rate and mean shoot number per explant was 63.33% ,91.11% and 5.86, respectively; however, the above indexes varied from genotype to genotype; scape was the best explant for inducing adventitious shoot in the three; 1/2MS ＋0.2 mg ·L^-1 NAA was optimal for root formation of regenerated shoots, with 81.11% rooting rate and 6.08 roots per shoot; the conclusion was that we successfully established plant regeneration of three genotypes, mean shoot number per explant was all more than 4 and Stelladaorp was the most, which was 6.88.