猪繁殖与呼吸综合征病毒不同毒株感染对猪外周血免疫细胞含量的影响

The Effects of Different PRRSV Strains Infection on the Peripheral Blood Immune Cells of Swine

采用猪繁殖与呼吸综合征病毒(PRRSV)变异株HN07-01和经典美洲株BJ-4人工感染2月龄仔猪,通过观察发病情况、检测外周血免疫细胞和PRRSV特异血清抗体水平,研究了不同毒株PRRSV的致病特性。结果表明:PRRSV变异株感染后能够引起高热症状;变异株HN07-01株感染后引起的外周血各类免疫细胞下降速度和下降程度显著高于BJ-4株。提示PRRSV变异株引起机体的免疫抑制明显强于BJ-4株;PRRSV特异血清抗体结果表明:变异株和BJ-4株均能快速诱导机体产生抗体。

In this study,2-month-old piglets were inoculated with porcine reproductive and respiratory syndrome virus（PRRSV） variant strain HN07-01 and classical strain BJ-4,respectively.To investigate the pathogenicity of different PRRSV strains,the clinical symptoms was observed and the peripheral blood immune cells and serum PRRSV-specific antibodies were detected.The results showed that the high fever was induced by PRRSV variant strain.Peripheral blood immune cells in HN07-01 infected piglets decreased significantly than that in BJ-4 strain,which indicated the severer immune suppression induced by PRRSV variant.The result of PRRSV-specific antibody detection showed that both the PRRSV variant and BJ-4 strain can induce antibody quickly.