甘糖酯体外对兔晶状体上皮细胞增殖的抑制作用(英文)

Inhibitory effect of propylene glycol mannate sulfate on growth of rabbit lens epithelial cells in vitro

目的:观察甘糖酯(propylene glycol mannate sulfate,PGMS)对囊袋培养的兔晶状体上皮细胞(RLEC)增殖、移行的抑制作用。方法:新鲜处死的兔眼,模拟白内障囊外摘除术,体外环形撕囊,水核分离,去除核与皮质,游离晶状体囊袋并固定,建立RLEC体外培养的囊袋模型。实验组分别用0.2,0.4,0.8g/L的PGMS浸泡晶状体囊袋,作用时间分别为2,5,10min,同时设空白对照组。上述处理后,囊袋标本置含50mL/L胎牛血清的DMEM营养液中培养。7d后观察RLEC增殖、移行情况并行组织病理学、电镜检查。结果:实验组随着PGMS浓度的增加和作用时间的延长,细胞增殖、移行的速度明显减慢,其中浓度为0.8g/L,作用时间5min和10min两组,显著抑制RLEC生长(P<0.05)。对照组后囊上RLEC增殖、移行速度较快,培养7d后所有标本后囊细胞覆盖率达100%。电镜下对照组细胞结构完整;0.4g/L及0.8g/L实验组细胞退变明显。结论:甘糖酯能有效抑制体外培养的RLEC的增殖和移行。

AIM：To investigate the inhibitory effect of rabbit lens epithelial cell（RLEC）survival and growth by propylene glycol mannate sulfate（PGMS）on the rabbit capsular bag in vitro.·METHODS：Capsular bags were prepared from rabbit eyes after extracapsular cataract extraction（ECCE） and incubated in 0.2,0.4,0.8g/L PGMS in 2,5,10 minutes incubation periods.After treatment,the capsular bags were cultured for 7 days in Dulbecco minimum essential medium（DMEM） supplemented with 50mL/L fetal calf serum（FCS）.The specimens were examined with light microscopy and transmission electron microscopy（TEM）.Capsular bags without receiving PGMS only served as controls.·RESULTS：PGMS inhibited the proliferation of RLEC in the manner of concentration and time dependentment.At the threshold protocol of incubation in PGMS at 0.8g/L for 5 or 10 minutes,proliferative activity of cells were largely arrested and nearly no RLEC was seen on the posterior capsule（P〈0.05）.Control group had no effect on structure and proliferative activity of RLEC,and the growth proceeded rapidly so that the posterior capsule were totally covered by a confluent monolayer of cell by the end of 7 days.Under TEM,the cells in the control group were tightly arrayed with clearly defined cellular boundary and structure;while cellular deformity and undefined intracellular structure could be seen in the 0.4g/L and 0.8g/L experimental groups.·CONCLUSION：PGMS can effectively inhibit the proliferation of RLEC.