摘要
背景与目的:近来研究表明,胶质瘤中一小群表达干细胞标志的细胞是胶质瘤发生及发展的根源。本文探讨干细胞标志物CD133和Nestin在不同恶性程度人脑胶质瘤组织中的表达情况及其与预后的关系。方法:应用实时荧光定量PCR方法定量检测77例不同恶性程度胶质瘤组织中CD133和Nestin基因的表达情况,并与肿瘤的恶性程度进行相关分析;探讨肿瘤组织中上述基因的表达水平与患者预后的关系。结果:在不同恶性程度胶质瘤组织中,CD133及Nestin的表达存在显著性差异(P<0.05),CD133和Nestin的表达情况与肿瘤的恶性程度呈正性相关(P<0.05);单因素预后分析显示CD133和Nestin的表达水平与预后相关(P<0.05),CD133和Nestin基因的高表达预示一个较短的生存时间;多因素Cox比例风险回归模型分析显示,CD133是独立于病理级别及其他临床变量的预后因子。结论:检测胶质瘤组织中CD133和Nestin的表达水平有助于评价肿瘤的生物学行为和患者的预后。
BACKGROUND ＆ OBJECTIVE： Accumulating evidence suggests that a small population of cells expressing stem cell markers are responsible for the initiation and progression of glioma. In this study, we investigated the expression of stem cell markers CD133 and Nestin in gliomas of different malignancy and its correlation with patients＇ prognosis. METHODS： The expression levels of CD133 and Nestin gene of 77 gliomas of differernt pathological grades were quantified using real-time quantitative PCR. The correlation between the expression levels and malignancy of the tumors were analyzed. Moreover, the ton＇elation of expression levels of stem cell markers with patients＇ survival time was analyzed. RESULT： Both CD133 and Nestin expression were significandy different between low-grad and high-grade gliomas. Positive correlation between the expression levels and malignancy （P〈 0.05） was observed. In univariate prognostic analysis, both CD133 and Nestin expression levels correlated the patients＇ prognosis （P〈 0.05）. A high expression level was significant for poor prognosis. According to the muhiparametric Cox＇s proportional hazardregression model analysis, CD133 was a prognostic marker independent of pathological grade and other clinical variables in the study. CONCLUSIONS： Detecting the expression levels of CD133 and Nestin will be helpful to evaluate the biological behaviors of gliomas and prognosis in the patients.
出处
《中国神经肿瘤杂志》
2009年第3期175-179,共5页
Chinese Journal of Neuro-Oncology
基金
国家自然科学基金资助项目(30772241)
卫生部科研课题(WKJ2005-2-031)
