乙型肝炎后肝硬化中医证候与白细胞介素10基因多态性的关联

Relationship between gene polymorphisms of interleukin-10 and syndrome types of traditional Chinese medicine in posthepatitis B cirrhosis

目的:探讨白细胞介素10(interleukin-10,IL-10)基因的单核苷酸多态性(single-nucleotide polymorphism,SNP)与乙型病毒性肝炎(乙肝)后肝硬化中医证候的关联。方法:运用聚合酶链反应限制性片段长度多态性(polymerase chain reaction-restriction fragment lengthpolymorphism,PCR—RFLP)和聚合酶链反应连接酶检测(polymerase chain reaction—ligase detection reac—tion,PCR—LDR)相结合的测序方法,检测226例乙肝后肝硬化患者 IL-10-592 A/C、-819 C/T 和 -1082 G/A位点的多态性,分析其基因型和等位基因频率的分布,以及这3个 SNP 位点与乙肝后肝硬化中医证候之间的关系。结果:IL-10-819位点 C 等位基因在脾虚湿盛证出现的频率明显高于非脾虚湿盛证(P<0.001),TT 基因型在肝气郁结证出现的频率明显高于非肝气郁结证(P<0.05)。IL-10-592 A/C 和-1082 G/A 位点的多态性与乙肝后肝硬化中医证候之间未发现有显著的关联(P>0.05)。结论:IL-10-819位点 C 等位基因可能与乙肝后肝硬化患者形成脾虚湿盛证相关,而其 TT 基因型可能与肝硬化患者形成肝气郁结证相关。

Objective： To investigate the relationship between single-nucleotide polymorphisms （SNPs） of interleukin-10 （IL-10） and syndrome types of traditional Chinese medicine （TCM） in posthepatitis B cirrhosis. Methods： The genotypes of IL-10-592 A/C, -819 C/T and -1082 G/A sites were determined by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）, and polymerase chain reaction-ligase detection reaction （PCR-LDR） combined with the sequencing analysis in 226 cases of posthepatitis B cirrhosis. The genotype and allele frequency distribution, and the relationship between the SNPs and TCM syndromes were analyzed. Results： The frequency of allele C at IL-10-819 point in spleen deficiency with overabundance of dampness syndrome was significantly higher than that in non-spleen deficiency with overabundance of dampness （P〈0.01） syndrome, and genotype TT in liver stagnation syndrome was significantly higher than that in non-liver stagnation syndrome （P〈0.05）. There was no significant relationship between the polymorphisms of IL-10-592 A/C, -1082 G/A and the TCM syndromes in posthepatitis B cirrhosis （P〉0.05）. Conclusion： In patients with posthepatitis B cirrhosis, allele C in IL-10-819 locus may be related to spleen deficiency with overabundance of dampness syndrome, and TT genotype in IL-10-819 locus may be related to liver stagnation syndrome.