经导管动脉栓塞对肝癌血管生成的影响

Transcatheter arterial embolization promotes angiogenesis in rabbit VX2 liver tumor

目的:探讨肝癌经导管动脉栓塞(transcatheter arterial embolization,TAE)对残存肿瘤血管生成的影响.方法:建立20只兔VX2肝癌模型.TAE处理组(n=10),接受150-250μm聚乙烯醇经导管肝动脉栓塞;对照组(n=10),接受蒸馏水经导管肝动脉注入模拟TAE.栓塞后6h或3d,处死实验兔进行肿瘤组织取样,采用免疫组织化学方法评价样本的血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达和微血管密度(microvessel density,MVD),微血管标记采用CD31抗体,实时PCR检测VEGFmRNA表达.结果:TAE处理组肿瘤呈不完全性中央坏死,坏死区周围可见肿瘤细胞存活.TAE处理组肿瘤的VEGF蛋白、mRNA水平和平均MVD均显著高于对照组(P=0.001,0.000,0.001).VEGF蛋白水平与MVD呈显著性正相关(r=0.677,P=0.001).结论:肝癌TAE可激活残存肿瘤血管生成,VEGF在TAE相关性血管生成中发挥重要调节作用.

AIM：To investigate the effect of transcatheter arterial embolization （TAE） on angiogenesis in rabbit VX2 liver tumor.METHODS：VX2 tumors were implanted in the liver of 20 New Zealand White rabbits.The animals were then divided into two groups：rabbits undergoing TAE with 150-250-μm polyvinyl alcohol particles （TAE group,n=10） and those undergoing sham embolization with distilled water （control group,n=10）.The animals were sacrificed six hours or three days after TAE,and tumor samples were harvested.Immunohisto-chemistry was performed to evaluate vascular endothelial growth factor （VEGF） protein expression and microvessel density （MVD）. Realtime reverse transcription-polymerase chain reaction （RT-PCR） was performed to examine the expression of VEGF mRNA. RESULTS： TAE-treated tumors showed in- complete central necrosis with residual viable tumor cells in the periphery. The levels of VEGF protein and mRNA and mean MVD were significantly higher in the TAE group than in the control group （P = 0.001, 0.000 and 0.001, respectively）. The expression level of VEGF protein was positively correlated with MVD in residual viable tumor cells （r = 0.677, P = 0.001）. CONCLUSION： TAE can promote angiogenesis in residual rabbit VX2 liver tumor after TAE perhaps in a VEGF-dependent manner.