乳酸菌载体pMG36e的应用现状

Application of Lactic Acid Bacteria Vector pMG36e

乳酸乳球菌通用表达质粒pMG36e是一个经典的人工构建的组成型表达载体,是以乳酸乳球菌乳脂亚种蛋白酶基因的转录和翻译信号为基础构建而成的。它包含一个强启动子,能够在多种细菌中表达外源蛋白。已用于研究细菌素作用机制,乳酸菌基因工程菌株的改造以及口服疫苗的开发等,应用领域十分广泛,已成为乳酸菌基因工程研究的重要工具质粒之一。现主要从载体构成、基因表达与食品级载体改造等三方面的应用对其进行综述,旨在为该质粒今后研究提供资料。

Plasmid pMG36e, based on protease gene transcription and translation signals from Lactococcus lactis cremoris, is a typical artificial constitutive expression vector as a main expression system in Lactococcus. It has a strong promoter and has been used for expression of heterologous protein such as enzyme in a variety of bacteria. In recent years, it was used to investigate the mechanism of bacteriocin as an intermediate vector, to construct lactic acid bacteria genetic strains and develop oral vaccine, even in gene therapy study, pMG36e has been used so widely in multi-disciplinary applications, it becomes one of the most important plasmid in the lactic acid bacteria genetic engineering. The basic plasmid composition, structure, and application of pMG36e in gene expression and food-grade vector construction were reviewed to provide reference for future research.