一个番茄细胞分裂素受体类激酶基因的生物信息学分析

Bioinformatics of A Cytokinin Receptor Like Kinase Gene in Tomato(Lycopersicum esculentum L.)

利用生物信息学的方法从番茄的基因组中解析得到一个与拟南芥渗透胁迫相关的细胞分裂素受体类激酶HK1高度同源的基因,命名为LeHK1,并对它的序列结构、蛋白基序、启动子区的顺式元件和基因的遗传进化进行了分析。结果显示,番茄基因LeHK1编码的蛋白具有组氨酸蛋白激酶的结构特征,而且在进化上与拟南芥的渗透胁迫感受基因AtHK1分属同一个类群。从基因顺式反应元件分析来看,番茄基因LeHK1具有多个响应不同环境信号如热、干旱、激素信号的顺式元件,预示这个基因在环境响应和植物生长发育过程中具有重要功能。

Through bioinformatic method and datamining, a tomato homolog of arabidopsis histidine kinase gene HK1 which encodes a cytokinin recepter like protein, was isolated and named LeHK1. The structure of LeHK1 sequence and its putative coding protein, aslo its cis-elements in promoter regioin and phylogenetic relasionship with other plants HKs was analyzed. The results showed that LeHK1 possesed the character of histidine kinase, and it was clustered in the same group with AtHK1. According to the result of cis-elements analysis, tomato HK1 has muptiple cis-elements responsive to different environmental factors such as heat, drought, and hormones. This suggests that LeHK1 may play key roles in response to stresses and development.