摘要
从土壤中筛选出一株产纤维素酶的细菌B1,通过形态观察、生理生化特征及16S rDNA同源性序列分析,鉴定为蜡样芽孢杆菌(Bacillus cereus)。经微波-硫酸二乙酯复合诱变筛选出正突变株B1-1,其纤维素酶酶活达到0.373 U/mL,经5次传代后,该菌株遗传性状稳定,且胃肠道耐受性良好;B1-1菌株最佳产酶条件为20 g/L麦芽糖,2.5 g/L硫酸铵,pH值为5.0,37℃下培养36 h。
Strain B1 with cellulase activity was screened from soil, which was identified as Bacillus cereus according to the characteristics of morphology, physiology and biochemistry tests and the comparison of 16S rDNA sequence. Strain B1-1 with high cellulase activity was filtrated by microwave and DES mutagenesis, and its cellulase activity reached 0. 373 U/mL. Strain B1-1 was genetically stable after five subcultures and well survived in the gastrointestinal tract. The optimum conditions for cellulase production were 20 g/L maltose, 2. 5 g/L ammonium sulphate, pH 5.0 and culturing for 36 h at 37 ℃.
出处
《合肥工业大学学报(自然科学版)》
CAS
CSCD
北大核心
2009年第11期1730-1734,共5页
Journal of Hefei University of Technology:Natural Science
基金
安徽省重点科研计划资助项目(07020303053)
安徽省企业技术创新资助项目(2008991)
关键词
纤维素酶
16S
RDNA
复合诱变
产酶条件
益生菌
cellulase
16S rDNA
composite mutation
condition of cellulase production
probiotics