用均匀设计优选香附SFE-CO_2萃取后药渣的半仿生法工艺条件

Technical condition of semi-bionic extraction in optimizing formula of Rhizoma Cyperi that had been extrscted by SFE-CO_2 through homogeneous design

目的:优选香附经SFE-CO2萃取后药渣的半仿生法工艺条件。方法:将香附SFE-CO2萃取后的药渣,用均匀设计UL(91×33)表,在煎提温度、煎提用水量、滤过、浓缩等条件相同的情况下,以相对分子质量≤1 000提取物、HPLC总面积、干浸膏为指标,综合评判,优选其提取的工艺条件。结果:3次煎提用水的pH依次为2.005 3,6.508 2,8.945 6,提取总时间为3.912 7 h。结论:结合生产实际,确定3次煎提用水的pH依次为2.0,6.5,9.0,提取时间依次为2.0,1.0,1.0 h。

Objective：To optimize the extraction condtions in the modification of Rhizoma Cyperi, which had been extrscted by the technique of supercritical CO2 extraction. Method：The semi-bionic extraction（SBE） conditions were optimized through homogeneous design while the extracts（ ≤1 000） ,the total area of HPLC and dry extracts were adopted as markers. Result：The optimized SBE extraction conditions are the following： pH in first extraction was adjusted to 2. 005 3. And then pH in second and third extraction adjusted to 6. 508 2 and 8. 945 6, time of the whole extracted process was 3. 912 7 h. Conclusion：Combine the faction of production,we make sure the conclusion is pH in first extraction was 2. 00, pH in second and third extraction adjusted to 6. 50 and 9. 00, duration of run was 2. 0 h, 1.0 h and 1.0 h, respectively.