脐血干细胞培养过程中有核红细胞数量变化的实验研究

Experimental research on changes of amount of nuclear red blood cells in cultivation of cord blood stem cells

目的:对脐血干细胞进行液体和半固体细胞培养观察、记录有核红细胞增殖情况。方法:对脐血干细胞进行液体和半固体细胞培养,用流式细胞仪观察、记录有核红细胞增殖情况。结果:脐血液体培养经流式细胞仪检测,NRBC得率最大为20.0%,平均7.3%。结论:脐血干细胞培养可以获得较高的NRBC含量。

Objective： To cultivate and observe the cord blood stem cells, record the proliferation of nuclear red blood cells in umbilical cord blood. Methods ： The cord blood stem cells were cultivated by liquid and half solid method, the proliferation of nuclear red blood cells in umbilical cord blood was observed by flow cytometry, then recorded. Results： The highest gain rate of nuclear red blood cells was 20.0% , and 7. 3% on average. Conclusion： The cultivation of cord blood stem cells can get high level of nuclear red blood cells.