镍污染区大气PM2.5对大鼠血清白介素-6和C反应蛋白水平的影响

Effect of PM2.5 in Ambient Air of the Nickel Contaminated Area on Inflammatory Factors in Serum of Rats

目的探讨镍污染区大气PM2.5对大鼠血清白介素-6(IL-6)和C反应蛋白(CRP)水平的影响。方法于2007年6—7月和12月在镍污染区和对照区进行两次PM2.5采集,对其浓度及其中7种元素含量进行分析。选取42只成年雄性SPF级Wistar大鼠,随机分为生理盐水空白对照组、镍污染区及对照区PM2.5低(1.6mg/kg)、中(8.0mg/kg)和高(40.0mg/kg)剂量组,每组6只,经气管分别一次性滴注PM2.5悬液和生理盐水。染毒24h后处死大鼠,检测大鼠血清中IL-6、CRP水平。结果镍污染区和对照区大气PM2.5浓度差异无统计学意义(P>0.05),镍污染区和对照区PM2.5中镍平均浓度相差近48.75倍,差异有统计学意义(P<0.05)。PM2.5染毒后大鼠血清中IL-6含量与染毒剂量呈正相关,镍污染区和对照区各中、高剂量组与空白对照组差异均有统计学意义(P<0.05);大鼠血清中CRP含量也均有所升高,镍污染区与对照区之间的差异在高剂量组有统计学意义(P<0.01)。结论两监测区大气PM2.5染毒均可造成大鼠急性炎性反应,镍污染区PM2.5染毒后大鼠血清中IL-6与CRP水平均高于对照区,其中镍可能起到了关键性作用。

Objective To investigate and compare the effects of PM2. 5 in ambient air samples collected from nickel and nonickel contaminated areas on inflammatory factors in the serum of rats. Methods The air PM2.5 of the nickel-contaminated area and the control area were collected and detected for some major elements. Forty-two male Wistar rats were randomly divided into 10 groups, 6 in each, which were respectively instilled with saline, suspended PM2.5 fluid of the nickel-contaminated area and the control area of 3 different doses （1.6,8.0,40.0 mg/kg） through the trachea. The rats were sacrificed 24 hours after being treated with PM2.5. The levels of IL-6, CRP in serum were examined. Results There was no significant difference in the level of PM2.5 between the two areas, the concentration of Ni in the nickel-polluted area was 48.75 times as that in the non-polluted area. A good dose-dependent manner was found in the test of IL-6 in nickel-contaminated groups. CRP was found significantly higher in the nickel-contaminated group and the control group than the saline control group （P〈0.05）.Among the groups of the nickel- contaminated area and the control area, significant differences were found in high dose groups （P〈0.01）.Conclusion PM2.5 can cause obvious inflammation in rats. Maybe for the different concentration of nickel, the significant difference of IL-6 and CRP levels in serum induced by PM2.5 between the nickel-polluted area and the non-polluted area is seen.