多因素环境改变对家兔血浆内皮素和一氧化氮水平的影响

Effect of Complex Environment Stimulus on the Plasma Endothelin and Nitric Oxide in Rabbits

目的探索高温、饥饿及多因素的环境改变对家兔血浆内皮素(ET)和一氧化氮(NO)影响。方法将90只健康中国白兔,随机分成心肌缺血组、心肌缺血低氧高温组、心肌缺血低氧组、心肌缺血饥饿组、心肌缺血低氧高温饥饿组、单纯饥饿组、单纯低氧组、单纯高温组和正常对照组,每组10只。以电灼心脏左前降支动脉实现心肌缺血,以吸入8.5%氮氧混合气体(91.5%为氮气,8.5%为氧气)实现低氧条件,高温条件为(42±2)℃。按各组的环境变化实验要求进行30min实验,然后抽取静脉血测各组血浆ET和NO含量。结果与对照组比较,除单纯饥饿组和单纯高温组外,其余6个组的ET含量均升高(P<0.05)。单纯高温组、心肌缺血饥饿组NO含量降低(P<0.05),其余各组均为降低趋势,但差异均无统计学意义(P>0.05)。结论多因素的环境变化对血浆ET和NO含量均发生不同程度的影响。

Objective To explore the environmental factors change on the plasma endothelin （ET） and nitric oxide （NO） in rabbits exposed to a complex environment： high temperature, hunger, and so on. Methods Ninety China rabbits were randomly divided into myocardial ischemia group, myocardial ischemia and hypoxia and high temperature group, myocardial ischemia and hypoxia group, myocardial ischemia and hunger group, myocardial ischemia and hypoxia and high temperature and hunger group, hunger group, hypoxia group, high temperatures group, control group, ten rabbits in each group. The animal myocardial ischemia was caused by fulgurating the left anterior descending branch artery in the heart. The animal hypoxia was caused by breathing in 8.5% nitrogen oxygen atmosphere （nitrogen content was 91.5%, oxygen content was 8.5% ）. The animals in the high temperature group were put into a high temperature [（42±2）℃] environment. The animals in the hunger group were treated with fasting and water deprivation for 24 h. Every animal has been taken in above-mentioned experiment environment for 30 min. Blood from jugular veins collected, and the contents of plasma ET and NO were determine. Results Compared with control group, except the hunger group and high temperature group, ET levels in the others six group were significantly increased （P〈0.05）. And the plasma NO levels in the high temperature group and the myocardial ischemia-hunger group were significantly decreased （P〈0.01）. There were downtrend in the plasma NO level in other exposure groups, but the distinction were not statistically significant. Conclusion The change of environment factors may have effects on the levels of ET and NO in plasma .