辣椒制品中对位红和苏丹红的高效液相色谱同时测定法

Simultaneous Determination of Para Red and Sudan Red in Capsicum Product by HPLC

目的探讨液相色谱对辣椒制品中对位红,苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的同时检测方法。方法用乙醚提取后于旋转蒸发仪上浓缩至1 ml,用甲醇定容至5 ml,进液相色谱分析,以保留时间定性,峰面积定量。结果在方法选定的条件下,对位红,苏丹红(Ⅰ、Ⅱ、Ⅲ、Ⅳ)不同水平加标回收率范围为:对位红为97.3%～103.0%,苏丹红Ⅰ为96.8%～102.0%,苏丹红Ⅱ为96.5%～102.0%,苏丹红Ⅲ为93.5%～105.0%,苏丹红Ⅳ为92.3%～102.0%。结论该方法简便、易行,适用于辣椒制品中对位红、苏丹红的测定。

[Objective]To investigate the simultaneous determination of Para red and Sudan （I,II,Ⅲ,Ⅳ）. [Methods]The product was distilled by aether and condensed to lml on the rotary evaporator, then magnified to 5ml in volume with methanol for HPLC analysis. The analysis was qualitative with retained time and qualitative of the peak area. [Results]Under the conditions of the method chosen, the recovery of standard addition of Para red and Sudan （I,II,III,Ⅳ） were 97.3% - 103. 0%, 96. 8%- 102. 0%, 96. 5% - 102. 0%, 93.5% - 105. 0% and 92.3% - 102. 0%, respectively. [ Conclusion]This method is simple, feasible and suitable for measurement of Para red and Sudan rod in the capsicum product.