调节性T细胞在原发性胆汁性肝硬化中的意义

The significance of T regulatory cells in primary biliary cirrhosis

目的研究调节性T细胞在原发性胆汁性肝硬化（PBC）中的特点，分析调节性T细胞在PBC发病机制中的意义。方法应用流式细胞技术分别检测27例PBC和14名健康对照外周血中调节性T细胞细胞含量；采用实时荧光定量聚合酶链反应（PCR）技术检测人叉头型基因p3（Foxp3）mRNA表达的差异；采用免疫组织化学检测肝脏Foxp3＋T细胞的表达。结果调节性T细胞在PBC外周血明显降低（P〈0．01）；Foxp3的mRNA水平差异无统计学意义（P〉0．05）；肝脏中Foxp^3＋调节性T细胞细胞的浸润程度随病理阶段和汇管区炎细胞浸润程度而变化。结论PBC外周血调节性T细胞下降，肝脏局部调节性T细胞增加，可能是自身免疫反应中局部炎症导致外周调节性T细胞迁移的结果。

Objective To-study the characteristics of T regulatory cells （Treg） in primary biliary cirrhosis （PBC）, and to analyze the significance of Treg in PBC. Methods Peripheral blood Treg cells of 27 PBC patients and 14 healthy controls were detected by tricolour flowcytometry. Simultaneously the expression of forkhcad box p3 （Foxp3） mRNA was determined by real-time RT-PCR assay. The expression of Foxp3 positive T cells in liver was exmined by immunohistochemistry. Results The percentage of Treg in the peripheral blood was lower in PBC （P〈0.01） than in healthy control group. The mRNA expression of Foxp3 had no significant difference （P〉0.05） between the two groups. The infiltration of Foxp3 positive T cells in liver tissue was different in different pathological stages and different degree of inflammation of the portal area. Conclusion The possible reason for Treg decreasing in the peripheral blood of PBC and Treg increasing in local inflammatory portal area of liver is the result of local inflammation which then lead to migration of peripheral Treg in autoimmunity.