摘要
目的:探讨NADPH氧化酶抑制剂apocynin对1型糖尿病小鼠心肌组织IL-1β、IL-18、TNF-α和COX-2表达的影响及其机制研究。方法:C57小鼠腹腔注射STZ建立1型糖尿病动物模型,44只8周龄雄性小鼠分为对照组(sham,n=6)、apocynin对照组(Apo,n=6)、1型糖尿病组(STZ,n=16)及1型糖尿病apocynin治疗组(STZ+Apo,n=16)。实验于8周末结束,运用超声心动图检测小鼠心脏功能,以实时定量RT-PCR技术测定心肌组织IL-1β、IL-18、TNF-α和COX-2mRNA表达,以Western blotting检测心肌组织磷酸化Akt表达。结果:1型糖尿病小鼠心脏功能降低伴随心肌组织IL-1β、IL-18、TNF-α和COX-2 mRNA表达增加,STZ+Apo组小鼠心脏功能增高伴随心肌组织IL-1β、IL-18、TNF-α和COX-2 mRNA表达减少;1型糖尿病小鼠心肌组织磷酸化Akt显著减少,STZ+Apo组心肌组织磷酸化Akt明显增加。结论:Apocynin提高1型糖尿病小鼠心脏功能伴随显著减少心肌组织IL-1β、IL-18、TNF-α和COX-2mRNA的表达,其机制可能与增加心肌组织磷酸化Akt表达密切相关。
AIM : This study was to investigate the effects of apocynin on the expression of IL -1β, IL - 18, TNF -α and COX - 2 mRNA in the diabetic cardiomyopathy heart. METHODS : Diabetes was induced in 8 - week old C57 mice with one intraperitoneal injection of streptozotocin (STZ, 100 mg/kg). The experiments were divided into 4 groups : sham ( n = 6 ) , apocynin control ( Apo, n = 6 ) , diabetic cardiomyopathy ( STZ, n = 16 ) and apocynin treatment (STZ + Apo, n = 16). At the end of 8 weeks after induction of diabetes, cardiac functions were evaluated by echocardiography. Cardiac tissue was analyzed for the expression of IL -1β, IL - 18, TNF -α, COX - 2 mRNA and phosphorylated Akt by real - time RT - PCR and Western blotting, respectively. RESULTS : The expression of IL -1β, IL - 18, TNF -α and COX- 2 mRNA increased in diabetic cardiomyopathy heart accompanied with cardiac dysfunction. Cardiac functions were improved with decreased levels of IL -1β, IL - 18, TNF -α and COX - 2 mRNA expression in apocynin - treated heart. Compared with reduction of phosphorylated Akt, apocynin significantly increased phosphorylation of Akt in diabetic cardiomyopathy heart. CONCLUSION: Apocynin decreases cardiac IL -1β, IL - 18, TNF -α and COX -2 mRNA expression accompanied with improved cardiac function, which associates with the increase of phosphorylated Akt in diabetic cardiomyopathy heart.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2009年第11期2109-2112,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30772021)
教育部新世纪优秀人才计划资助项目(No.NCEF-06-0354)
上海市浦江人才计划资助项目(No.09PJ148400)