碱性成纤维细胞生长因子对癫持续状态大鼠海马谷氨酸和γ-氨基丁酸的影响

Effects of basic fibroblast growth factor on the content of glutamic acid and aminobutyric acid in the hip-pocampus of rats following pentylenetetrazol-induced status epilepticus

目的通过戊四氮癫持续状态大鼠模型,观察碱性成纤维细胞生长因子(bFGF)对海马组织内主要的兴奋性氨基酸与抑制性氨基酸——谷氨酸和γ-氨基丁酸动态变化的影响。方法建立60 d大鼠戊四氮(PTZ)诱导癫持续状态模型,生理盐水(NS)注射作为对照,皮下注射bFGF进行干预,分4组:即NS组、NS+bFGF组、PTZ组、PTZ+bFGF组。选择处理后第3、7、14天三个时间点进行观察,采用高效液相法检测海马组织谷氨酸和γ-氨基丁酸含量。结果发作后3、7、14 d PTZ组海马组织谷氨酸较NS组有显著升高(P<0.01),以发作后14 d升高更为明显,PTZ+bFGF组各时间点谷氨酸较PTZ组下降(P<0.05);γ-氨基丁酸含量在PTZ组各时间点亦大于NS组(P<0.05),以发作后14 d升高较为显著;PTZ+bFGF组发作后各时间点γ-氨基丁酸较PTZ组差异无统计学意义(P>0.05)。结论大鼠癫持续状态后一定时间内海马谷氨酸和γ-氨基丁酸增加,bFGF能够降低大鼠癫发作后异常增加的谷氨酸含量。

Objective To study the effect of basic fibroblast growth factor （bFGF） on the content of glutamic acid and aminobutyric acid in rat hippocampus following pentylenetetrazol-indueed status epilepticus. Methods Postnatal day 60 （P60） Sprague Dawley （SD） rats received pentetrazole （PTZ） intra-abdominaly to make the model of status epilepticus （SE）. The rats were divided randomly into PTZ group, PTZ ＋ bFGF group, normal saline （NS） group and NS ＋ bFGF group. Each group was examined on the third day （d3）, the seventh day （d7） and the fourteenth day （d14） after SE. The content of glutamie acid and aminobutyrie acid （GABA） in homogenized brain tissue of the rats was detemined with high performance liquid chromatography. Results Content of glutamic acid was higher on d3, d7 and d14, especially higher on d14, after SE developed in PTZ group than that in NS group （P 〈 0.01） , the content of glutamic acid in PTZ ＋ BFGF group was lower than that in PTZ group but was higher than that in NS group （P 〈 0.05）. Content of GABA also increased after sizure （P 〈 0.05）, while there was no significant difference between PTZ group and PTZ ＋ bFGF group （P 〉 0.05 ）. Conclusions Content of both glutamie acid and GABA increased after SE developed for a period of time. bFGF can decrease the unusual increased content of glutamic acid in hippocampus.