耻垢分枝杆菌疫苗对小鼠巨噬细胞产生一氧化氮的影响

Effect of Mycobacterium Smegmatis Vaccine on the Level of Nitric Oxide Produced by Peritoneal Macrophages in Mice

目的探讨耻垢分枝杆菌疫苗对小鼠腹腔巨噬细胞产生一氧化氮的影响。方法将Balb/c小鼠随机分成生理盐水对照组和耻垢分枝杆菌疫苗低、中、高剂量组,分别注射生理盐水和不同剂量的耻垢分枝杆菌疫苗。取小鼠腹腔巨噬细胞体外培养,化学显色法测定培养上清中一氧化氮的含量。结果生理盐水对照组和耻垢分枝杆菌疫苗低、中、高剂量组产生的一氧化氮分别为(3.50±3.11)、(16.63±6.47)、(13.97±6.20)和(7.55±2.26)ng/ml,不同剂量疫苗免疫小鼠产生的一氧化氮水平均显著高于对照组(P<0.01)。结论耻垢分枝杆菌疫苗可促进小鼠巨噬细胞产生一氧化氮。

Objective To study the effect of Mycobacterium smegmatis vaccine on the level of nitric oxide （NO） produced by peritoneal macrophages in immunized mice. Methods Balb/c mice were randomized into low-dose,middle-dose,and high-dose groups （injected with different doses of Mycobacterium smegmatis vaccine） and a control group （injected with normal saline）. Then the peritoneal macrophages were cultured with lipopolysaccharide in vitro. The supernatants were collected and the concentrations of NO were analyzed through the reaction with Griess reagents. Results The levels of NO produced by the peritoneal in the control group, low-dose group, middle-dose group, and high-dose group were （3.50± 3.11 ） macrophages , （16.63± 6.47）, （13.97± 6.20）, and （7.55 ±2.26） ng/ml, respectively. The levels of NO in all dosing groups were significantly different from that in control group （P 〈 0.01 ）. Conclusion Mycobacterium smegmatis vaccine can promote the peritoneal macrophages to produce NO in mice.