鸦胆子油乳对宫颈癌SiHa细胞的抑制

Brucea javanica oil emulsion inhibits peoliferation of cervical cancer SiHa cells

目的:探讨鸦胆子(Brucea javanica)油乳在体外对宫颈癌SiHa细胞的抑制作用及其作用机制。方法:以鸦胆子油乳(2.5、5.0、10、20、40、80μg/ml)作用于SiHa细胞24、48、72h,以MTT法检测鸦胆子油乳对SiHa细胞增殖的抑制作用;透射电镜观察细胞的超微结构;琼脂糖凝胶电泳观察SiHa细胞基因组DNA片段化改变;流式细胞术测定AnnexinV/PI双染色后SiHa细胞凋亡率。结果:MTT结果显示,鸦胆子油乳以剂量和时间依赖方式对SiHa细胞增殖有明显的抑制作用,其中80μg/ml药物作用72h时的抑制率可达92.43%;20μg/ml药物作用后,透射电镜下可观察到细胞出现凋亡小体,琼脂糖凝胶电泳显示细胞凋亡特征性的DNA片段化"梯状"条带;流式细胞仪检测显示,10、20、40μg/ml药物组作用48h后凋亡率分别为(8.02±2.41)%、(51.60±7.67)%、(77.22±5.80)%。结论:鸦胆子油乳能够抑制SiHa细胞增殖,其作用机制可能为诱导SiHa细胞发生凋亡。

Objective：To investigate the inhibitory effect of Brucea javanica oil emulsion on cervical cancer SiHa cells in vitro and the related mechanisms. Methods： SiHa cells were treated with Brucea javanica oil emulsion（2.5, 5.0, 10, 20, 40, and 80 μg/ml）for 24, 48, and 72 h. The inhibitory effect of Brucea javanica oil emulsion on the proliferation of SiHa cells was examined by MTT; the ultrastructure of SiHa cells was observed by transmission electron microscope （TEM）; the DNA fragment of SiHa cells was detected by agarose gel electrophoresis; and the apoptosis of SiHa cells was examined by flow cytometry through Annexin V and PI staining. Results：MTT results showed that Brucea javanica oil emulsion significantly inhibited the proliferation of SiHa cells in a dose and timedependent manner. The inhibitory rate of SiHa cells reached 92.43% after treatment with 80 μg/ml Brucea javanica oil emulsion for 72 h. Apoptotic bodies and a characteristic apoptosis DNA ladder of SiHa cells were observed after treatment with 20 μg/ml Brucea javanica oil emulsion. Flow cytometry results demonstrated that the apoptosis rates of SiHa cells were （8.02±241）%, （51.60±7.67）%, and （77.22±580）% 48 h after treatment with 10, 20, and 40 μg/ml Brucea javanica oil emulsion for 48 h, respectively. Conclusion： Brucea javanica oil emulsion can inhibit the proliferation of SiHa cells, probably by inducing apoptosis of SiHa cells.