恶性胶质瘤中IL-13Rα2和PCNA的表达及其与病理特征和预后的关系

Expression of IL-13Rα2 and PCNA in malignant glioma and its relationship with pathological characteristics and prognosis

目的:检测人脑恶性胶质瘤组织中IL-13Rα2和增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达强度,分析其与患者临床病理特点和预后之间的关系。方法:选取2002-2006年温州医学院附属第一医院手术治疗的43例恶性胶质瘤标本;采用免疫组织化学法检测并用图像分析系统分析IL-13Rα2和PCNA在恶性胶质瘤组织中的表达,分析两者间的相关性;分析它们与临床特征和预后的关系。结果:(1)43例恶性胶质瘤标本有40例(93%)IL-13Rα2阳性表达和36例(84%)PCNA阳性表达;(2)IL-13Rα2和PCNA表达强度与肿瘤部位和肿瘤大小无相关性(P>0.05);在WHOⅢ级与Ⅳ级间两者表达强度差异均有统计学意义(P=0.031,P=0.002);在生存时间≤6个月与>6个月患者间IL-13Rα2表达强度差异有统计学意义(P=0.028)。(3)IL-13Rα2和PCNA的表达强度呈显著的正相关(r=0.653,P=0.000)。结论:人恶性胶质瘤组织中IL-13Rα2和PCNA均高表达,前者表达强度与肿瘤恶性分级和患者预后密切相关,在恶性胶质瘤的诊断和预后判断中具有潜在的临床应用价值。

Objective：To examine the expression of IL-13Rα2 and proliferating cell nuclear antigen （PCNA） in malignant glioma, and to investigate its relationship with clinical pathological characteristics and prognosis. Methods：Fortythree surgical samples of malignant glioma were obtained from First Affiliated Hospital of Wenzhou Medical College during 20022006. Expression of IL-13Rα2 and PCNA proteins was examined by immunohistochemistry staining, and the integrated optical density （IOD） was analyzed by image analysis system. The correlation between the expression of IL-13Rα2 with that of PCNA, and their relationship with clinical characteristics and prognosis of glioma were studied.Results： （1） The positive rates of IL-13Rα2 and PCNA in malignant glioma tissues were 93% （40/43） and 84% （36/43）, respectively. （2） No relationship of IL13Rα2 and PCNA expression with the location and tumor size of glioma was found （P〉0.05）. The expression of IL13Rα2 and PCNA in glioma was significantly different between Ⅲ grade and Ⅳ grade glioma tissues （P=0.031, P=0.002）. Patients who survived less than 6 months had a significantly higher expression of IL-13Rα2 than those who survived more than 6 months （P=0.028）. （3） The expression of IL-13Rα2 was positively correlated with that of PCNA （r=0.653, P=0.000）. Conclusion： IL-13Rα2 and PCNA are overexpressed in malignant glioma tissues, and IL-13Rα2 expression is correlated with differentiation grade and prognosis of glioma. IL-13Rα2 has potential roles in clinical diagnosis and prognositic evaluation of malignant glioma.