摘要
目的:探讨增殖细胞核抗原(Proliferating cell nuclear antigen,PCNA)在小鼠胚胎着床过程中的作用。方法:实时荧光定量聚合酶链反应(Real-time Fluorescence Quantitative PCR,Real-time FQ-PCR)技术检测未孕及孕2、3、4、5、6、7 d(分别记为d0、d2、d3、d4、d5、d6、d7)小鼠子宫内膜PCNA mRNA的表达;子宫角注射PCNA单克隆抗体观察胚泡着床数。结果:FQ-PCR测得随着小鼠妊娠天数的增加,PCNA mRNA表达量也逐渐增高,在孕d4、d5达到峰值,孕d6、d7逐渐下降。子宫角注射PCNA单克隆抗体后胚泡着床数明显减少。结论:PCNA在妊娠早期子宫内膜持续表达,可能参与了胚泡着床。
Objective:To investigate the effect of PCNA in the mouse uterus during embryo implantation.Methods:Real-time fluorescence quantitative PCR(FQ-PCR) was used to detect the PCNA mRNA of the mouse of unpregnancy,pregnancy at d2,d3,d4,d5,d6 and d7.PCNA monoclonal antibody was injected in horn of uterus at pregnant d3.Results: The expression of PCNA mRNA showed gradual hoist trend as days passed,and at pregnant d4,d5 it reached the maximum,at pregnant d6,d7 it showed gradual descent trend.The number of blastocysts in horn of uterus which was injected PCNA monoclonal antibody was smaller than in that of injected with Saline. Conclusion: PCNA is persistently expressed in mouse endometrium during the early pregnancy and maybe participates in the regulation process of mouse blastodysts implantation.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第10期1354-1357,共4页
Journal of Chongqing Medical University
关键词
PCNA子宫内膜
植入
FQ-PCR
子宫角注射
PCNA
Endometria
Implantation
Real-timefluoresc ence quantitative PCR(FQ-PCR)
Intrauterine injection