康复训练对脑缺血大鼠突触体素表达的影响

Effects of rehabilitation training on the expression of synaptophysin in the ischemic cerebral cortex

目的研究康复训练对脑缺血大鼠突触体素表达的影响。方法将80只SD大鼠随机分为脑缺血组、制动组、康复训练组及假手术组。脑缺血组、制动组及康复训练组大鼠均制成脑缺血动物模型，假手术组制模方法同上，但不阻断大脑中动脉血流。假手术组及脑缺血组大鼠于制模结束后均置于普通笼内饲养，制动组大鼠术后则置于网状笼内固定，康复训练组大鼠术后每天给予康复训练，包括滚笼、平衡木、转棒及网屏训练。于实验进行1，7，14及21d时各组分别取5只大鼠检测神经、运动功能以及大脑皮质突触体素表达水平。结果从术后第7天开始，康复训练组大鼠神经及运动功能均明显优于脑缺血组及制动组。假手术组大鼠脑皮质内可见突触体素免疫产物呈点状分布，密度较高；脑缺血组大鼠随缺血时间延长，其脑皮质内突触体素免疫产物逐渐减少，密度降低；制动组大鼠随缺血时间延长，其脑皮质内突触体素免疫产物减少程度更显著；康复训练组随缺血时间延长，其脑皮质内突触体素免疫产物减少幅度逐渐趋缓，明显高于制动组水平。结论脑缺血能显著降低实验大鼠脑皮质突触体素水平，康复训练能明显增强大鼠脑皮质突触体素表达，促进神经突触再生及神经、运动功能恢复。

Objective To study the effects of rehabilitation training on the expression of synaptophysin in the cortex after cerebral ischemia. Methods A model of focal cerebral ischemia was created in rats. The rats were divided into 4 groups at random 24 h after the cerebral ischemia was induced. The rehabilitation group was given balancing, grasping, rotation, walking and other training every day. An immobilization group was fixed in their cages. The model control group and cerebral ischemia group were kept in general cages, taking food and moving freely. Immunohistochemisty method was used to detect synaptophysin expression at the 1 st, 7th, 14th, 21st and 28th day after the cerebral ischemia was induced. Results In the model controi group, synaptophysin immunoreactive positive products were observed. In the cerebral ischemia group, the expression of synaptophysin decreased gradually. After rehabilitation training, synaptophysin immunoreactive positive products decreased slowly, but remained more abundant than among the immobilized rats. Conclusion After cerebral ischemia, the expression of synaptophysin decreases. This demonstrates that the synaptic terminals were less injured. Rehabilitation training can enhance synaptogenesis in the cortex after cerebral ischemia.