参松养心胶囊对Kv1.4钾电流特性的影响

Effects of shensongyangxin capsule on c-type Kv1.4 potassium channel

目的:探讨中药复方制剂参松养心胶囊对KV1.4钾通道C型(KV1.4△N)失活的影响。方法:将Kv1.4ΔNmRNA注射入非洲爪蟾卵母细胞中,使用双电极钳制法(Two electrodes voltage clamp TEV)观察参松养心胶囊对KV1.4ΔN电生理特性的影响。结果:参松养心胶囊对Kv1.4ΔN通道的峰电流有抑制作用,这种阻滞作用具有电压依赖性,随电位的升高而作用加强,符合单指数和线性关系。参松养心胶囊可加速KV1.4ΔN钾通道电流的失活过程,同时可使KV1.4ΔN通道失活后的恢复减慢。结论:参松养心胶囊能显著抑制KV1.4钾通道电流,这可能是其抗心律失常作用的机制之一。

AIM： To study the effects of shensongyangxin capsule, a traditional Chinese medicine, on c-type Kv1.4 channels in xenopus laevis oocytes by two-electrode voltage-clamp technique. METHODS： Defolliculated oocytes （stages V VI） were injected with transcribed cRNAs of ferret Kv1.4△N channels. During recording, oocytes were continuously perfused with control solution or shensongyangxin. RESULTS： Shensongyangxin decreased the currents during voltage steps in a voltage-dependent manner. The block increased with positive potentials and the voltage dependence of block matched the sum of monoexponentials and linear relationships. Shensongyangxin accelerated the inactivation of current during the voltage step and prolonged the time of recovery from the inactivation. CONCLUSION： These results suggest that shensongyangxin blocks the Kv1.4△N channel, which may underly its antiarrhythmic mechanisms.