相同条件下不同时间保存牛卵巢对卵母细胞体外成熟和体外受精成绩的影响

Effect of Various Storage Time of Ovaries on IVM and IVF of Oocytes Under the same Circumstances

本试验在相同条件下不同时间保存牛卵巢对印母细胞体外成熟和体外受精成绩的影响进行了初步探讨，结果24h保存组的体外成熟率55．5％低于0h保存组的成熟率86．6％，p＜0．01；由生发泡（GV）期到第一次成熟分裂中期的卵子比例24h保存组的38．9％显著高于0h保存组的10．7％，P<0．01；24h保存组的体外受精率47．4％极显著低于0h保存组的62．1％，P＜0．01。

This experiment was undertake to examine how did various storage time affect IVM and IVF of bovine oocytes under the same conditions. Ovaries were divided into two groups. Ovaries in group 1 had been stored for 24h, ovaries in group 2 weren't stored. As a result, the maturation rate of oocytes in group 1 (55. 5 % ) was significantly lower than that in group 2 (86. 6 %,p＜0. 01). The fertilization rate of oocytes in group 1 (47. 4% ) was significantly lower than that in group 2 (62. 1%,p＜0.01). But the rate of oocytes (38.9% ) that developed from the germinal vesicle stage to metaphase I stage in group 1 was significantly higher than that in group 2 (10. 7 %,p＜0. 01).