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我国生产用卡介菌的传代稳定性 被引量：6

Genetic Stability during Subculture of BCG for Vaccine Production in China
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摘要 目的研究我国生产用卡介菌上海株在传代过程中分子遗传学特性的稳定性。方法应用多重PCR法,对我国生产用卡介菌上海株工作种子批及其不同代次菌的各缺失区、插入序列IS6110及基因座SenX3-RenX3串联重复序列拷贝数进行检测。结果我国生产用卡介菌上海株的工作种子批及其第6、9、12代菌均缺失RD1、RD2,未缺失RD8、RD14、RD16、nRD18及RDDenmark/Glaxo;含有1个IS6110插入序列和3个SenX3-RenX3基因座串联重复序列。结论我国生产用卡介菌上海株在传12代以内,其分子遗传学特性是稳定的。 Objective To study the stability of molecular genetic characters during subculture of BCG China strain for vaccine production in China. Methods The region of deletion （RD）, copy numbers of IS6110 and variable number tandem repeat sequences （VNTRs） at SenX3-RenX3 locus in working seeds and various passages of BCG China strain were tested by multiplex PCR. Results Both RD1 and RD2 were deleted in working seeds and passages 6, 9 and 12 of BCG China strain, while RDS, RDI4, RD16, nRD18 and RDDenmark/Glaxo were undeleted. The working seeds and various passages of BCG China strain contained one IS6110 and 3 VNTRs at SenX3-RenX3 locus. Conclusion The molecular genetic characters of BCG China strain was stable within 12 passages.
作者 赵爱华 乔来艳 贾淑珍 寇丽杰 王国治
机构地区 中国药品生物制品检定所细菌一室
出处 《中国生物制品学杂志》 CAS CSCD 2009年第11期1102-1104,1112,共4页 Chinese Journal of Biologicals
关键词 卡介菌上海株 遗传学特性 传代 稳定性 BCG China strain Genetic character Subculture Stability
分类号 Q933 [生物学—微生物学]
  • 相关文献

参考文献16

  • 1Bedwe H J, Kairo SK, Behr MA, et al. Identification of substrains of BCG vaccine using multiplex PCR Vaccine, 2001, 19 (15-16): 2146-2151.
  • 2赵爱华,贾淑珍,寇丽杰,乔来艳,王国治.国内外卡介菌的分子遗传学特性[J].中国生物制品学杂志,2009,22(6):583-587. 被引量：7
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二级参考文献11

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共引文献6

同被引文献44

  • 1李国利,陈保文,庄玉辉,王国治,赵铭,沈小兵,孙桂芝.应用多重聚合酶链反应技术快速鉴定牛分支杆菌卡介苗株[J].中华结核和呼吸杂志,2003,26(11):701-703. 被引量：4
  • 2赵爱华,贾淑珍,乔来艳,寇丽杰,王国治.BCG-CpG-DNA制备、理化特性及免疫刺激活性的初步研究[J].微生物学免疫学进展,2005,33(1):39-43. 被引量：18
  • 3Behr M A, Wilson M A, Gill W P, et al. Comparative genomics of BCG vaccines by whole-genome DNA microarray [J]. Science, 1999, 284: 1520-1523.
  • 4Talbot E A, Willams D L, Frothingham R. PCR identification of Myeobaeterium bovis BCG [J] . J Clinical Microbiology, 1997, 35 (3): 566-569.
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  • 7WHO. WHO discussion on the improvement of the quality control of BCG vaccine, Quality Assurance and Safety of Biologicals [Z]. 7 June 2005.
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  • 9Knezevic I, Corbel MJ. WHO discussion on the improvement of the quality control of BCG vaccines.Pasteur Institute,Paris, France,7 June 2005 [ J ]. Vaccine, 2006, 24 ( 18 ):3874-3877.
  • 10Clarridge JE 3rd. Impact of 16S rRNA gene sequence analysis for identification of bacteria on clinical microbiology and infectious diseases [J]. Clin Microbiol Rev, 2004, 17 (4): 840- 862.

引证文献6

二级引证文献17

中国生物制品学杂志
2009年 第11期

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