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人工光照条件下深红红螺菌的氢代谢途径 被引量：1

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摘要 深红红螺菌(Rhodospirillum rubrum)在不同培养条件下分别有多种酶参与氢的代谢.为研究R.rubrum在人工光照条件下氢代谢途径及各代谢途径对光合产氢的贡献,分别构建了3个缺失突变株:Fe-固氮酶缺失单突变株、Fe-固氮酶和Mo-固氮酶双缺失突变株以及吸氢酶和Fe-固氮酶双缺失突变株.比较R.rubrum野生型菌株、吸氢酶缺失单突变株及所构建3个突变株的固氮酶活性及光合氢产量.结果表明,在人工光照条件下,Mo-固氮酶和Fe-固氮酶是R.rubrum产氢的关键酶;除Fe-和Mo-固氮酶外还有第3种途径参与氢代谢,该代谢途径产生的氢气量较小.Mo-固氮酶、Fe-固氮酶和第3种途径对光合产氢的贡献率分别为93.5%,4.9%和1.5%;吸氢酶消耗13.3%的氢气.甲酸裂解氢酶活性测定表明,第3种产氢途径并非由甲酸裂解氢酶介导,而可能是一种未知酶参与人工光照条件下R.rubrum的氢代谢.
作者 朱瑞艳 李季伦
机构地区 中国农业大学生物学院 燕山大学环境与化学工程学院 河北省生物新能源工程技术研究中心
出处 《科学通报》 EI CAS CSCD 北大核心 2009年第21期3320-3325,共6页 Chinese Science Bulletin
基金 国家高技术研究发展计划资助项目(编号:2006AA05Z108)
关键词 深红红螺菌 氢代谢途径 Mo-固氮酶 Fe-固氮酶 吸氢酶
分类号 Q936 [生物学—微生物学]
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参考文献25

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共引文献4

同被引文献9

  • 1沈永红,张彭湃.微生物生理学实验教学改革的几点体会[J].生物学通报,2005,40(11):40-42. 被引量：7
  • 2Moat A G,Foster J W,Specter M P.微生物生理学[M].4版.李颖,文莹,关国华,等译.李季伦,宋大康,校.北京:高等教育出版社,2009:336-339.
  • 3Wang Di,Zhang Yaoping,Welch Emily,et al.Elimination of Rubi-sco alters the regulation of nitrogenase activity and increases hydro-gen production in Rhodospirillum rubrum[J].International Journalof Hydrogen Energy,2010(35):7377-7385.
  • 4Zou Xiaoxiao,Zhu Yu,Pohlmann E L,et al.Identification and func-tional characterization of NifA variants that are independent of Gl-nB activation in the photosynthetic bacterium Rhodospirillumrubrum[J].Microbiology,2008(154):2689–2699.
  • 5Willey J M,Sherwood L M,Woolverton C J.Prescott,Harley,andKlein’s Microbiology[M].7th.New York:Mc Graw Hill HigherEducation,2008:103.
  • 6钱存柔,黄仪秀.微生物学实验教程[M].2版.北京:北京大学出版社,2008:105-107.
  • 7徐晖.改革实验教学 提高微生物学实验教学的质量[J].实验室研究与探索,2009,28(10):136-137. 被引量：18
  • 8ZHU RuiYan1,2,3 & LI JiLun1 1 College of Biological Sciences, State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100193, China,2 College of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, China,3 Engineering Research Center for Bioenergy of Hebei Province, Qinhuangdao 066004, China.Hydrogen metabolic pathways of Rhodospirillum rubrum under artificial illumination[J].Chinese Science Bulletin,2010,55(1):32-37. 被引量：1
  • 9邓百万,陈文强,彭浩,刘开辉,丁小维,解修超.基于能力培养的微生物实验教学手段与方法的改革研究[J].实验技术与管理,2011,28(2):7-10. 被引量：64

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科学通报
2009年 第21期

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