期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

蛋白酶激活受体在人单核细胞上的表达

Expressions of Protease Activated Receptors on Human monocyte.
下载PDF
导出
摘要 目的蛋白酶激活受体(protease activated receptors,PARs)表达于几乎所有的造血细胞上,PARs在单核细胞上的表达结果不一致,我们用高纯化的人外周血单核细胞研究PARs在单核细胞的表达。方法采用密度梯度离心法和磁激活细胞分选法分离人外周血单核细胞,采用反转录聚合酶链反应(RT-PCR)、流式细胞仪分析单核细胞PARs的表达情况。结果人外周血单核细胞表达PAR-1、PAR-3和PAR-4但不表达PAR-2蛋白质,单核细胞表达PAR-1和PAR-3,但是不表达PAR-2和PAR-4 mRNA。结论单核细胞表达PARs,这为进一步研究PARs在单核细胞的功能奠定了基础。 Objective To explore the expression of protease activated receptors （PARs） on human monoeytes. Methods Flow eytometry and RT - PCR was used to detect the expressions of PARs on human monocytes that purified by MACS. Results FACS analysis showed that monocytes expressed PAR - 1, PAR -3, PAR -4, but not PAR -2. RT- PCR revealed that monocytes expressed PAR -1 and PAR - 3, but not PAR - 2 and PAR - 4 mRNA. Conclusion PARs is expressed on human monoeyte cells,which may facilitate further investigation of the potential functions of PARs on monocytes.
作者 邹志强 李涛 陈镇奇 庄文 李廷俊 张锦忠 杨湘辉
机构地区 广东省汕头市中心血站 汕头大学医学院第一附属医院感染科
出处 《医学研究杂志》 2009年第10期34-36,共3页 Journal of Medical Research
基金 广东省汕头市2008年度重点科技计划项目(2008085)
关键词 蛋白酶激活受体 单核细胞 RT - NCR 流式细胞术 Protease activated receptors Monocyte RT - PCR Flow cytometry
分类号 R392.3 [医药卫生—免疫学] R978.1 [医药卫生—药品]
  • 相关文献

参考文献10

  • 10ssivsjaya VS, Bunnett NW. Protease activated receptors: contribution to physiology and disease. Physiol Rev,2004,84 (2) :579 - 621.
  • 2Howells GL, Kapas S, Macey MG. The protease - activated receptors and their cellular expression and function in blood - related ceils. Br J Haematol, 1998,101 : 1 - 9.
  • 3Johansson U, Lawson C, Dabare M,et al. Human peripheral blood monocytes express protease receptor - 2 and respond to receptor activation by production of IL -6, IL -8, and IL - 1β. J Leukoc Biol, 2005,78:967 - 975.
  • 4Colognato R, Slupsky JP, Jendrach M, et al. Differential expression and regulation of protease - activated receptors in human peripheral monocytes and monocyte - derived antigen - presenting cells. Blood, 2003,102:2645 - 2652.
  • 5Tao L, Wang H Y, He S H. Induction of Interleukin - 6 Release from Monocytes by Serine Proteinases and its Potential Mechanisms. Scan J Immuno1,2006, 64 : 10 - 16.
  • 6Steinhoff M, Buddenkotte J, Shpaeoviteh V,et al. Proteinase- Activated Receptors: Transducers of Proteinase - Mediated Signaling in Inflammation and Immune Response. Endoc Rev,2005, 26:1 -43.
  • 7Grage - Griebenow E, Flad HD, Ernst M. Heterogeneity of human peripheral blood monocyte subsets. J Leukoc Biol,2001, 69:11 -20.
  • 8Weber C, Belge KU, yon Hundelshausen P,et al. Differential chemokine receptor expression and function in human monocyte subpopulations. J Leukoc Biol,2000,67:699 -704.
  • 9Haskill S, Johnson C, Eierman D,et al. Adherence induces selective mRNA expression of monocyte mediators and proto - oncogenes. J Immanol,1988, 140:1690 - 1694.
  • 10王海燕,何韶衡,付意玲,方泽曼.人肺上皮细胞表达蛋白酶激活受体[J].第四军医大学学报,2005,26(17):1564-1566. 被引量：2

二级参考文献11

  • 1王海燕,何韶衡.蛋白酶激活受体2激动剂诱导上皮细胞分泌MCP-1[J].第四军医大学学报,2004,25(19):1782-1784. 被引量：8
  • 2Stellato C, Beck LA, Gorgone GA, et al. Expression of the chemokine RANTES by a human bronchial epithelial cell line: Modulation by cytokines and glucocorticoids [J]. J Immunol, 1995;155(1): 410-418.
  • 3Knight DA, Stewart GA, Thompson PJ. The respiratory epithelium and airway smooth muscle homeostasis: Its relevance to asthma [J]. Clin Exp Allergy, 1994;24(8):698-706.
  • 4De'ry O, Corvera CU, Steinhoff M, et al. Proteinase activated receptors: Novel mechanisms of signaling by serine proteases [J]. Am J Physiol, 1998;274 (6 Pt 1):C1429-C1452.
  • 5Ossovskaya VS, Bunnett NW. Protease-activated receptors: Contribution to physiology and disease[J]. Physiol Rev, 2004;84(2): 579-621.
  • 6Asokananthan N, Graham PT, Fink J, et al. Activation of protease-activated receptor (PAR)-1, PAR-2, and PAR-4 stimulates IL-6, IL-8, and prostaglandin E2 release from human respiratory epithelial cells[J]. J Immunol, 2002;168(7):3577-3585.
  • 7He S, Aslam A, Gaca MD, et al. Inhibitors of tryptase as mast cell-stabilizing agents in the human airways: Effects of tryptase and other agonists of proteinase-activated receptor 2 on histamine release [J]. J Pharmacol Exp Ther, 2004;309(1):119-126.
  • 8O'Brien PJ, Molino M, Kahn M, et al. Protease activated receptors: Theme and variations [J]. Oncogene, 2001; 20(13):1570-1581.
  • 9Macfarlane SR, Seatter MJ, KANKE T, et al. Proteinase-Activated Receptors[J]. Pharmacol Rev, 2001;53(2): 245-282.
  • 10Coughlin SR. Protease-activated receptors in vascular biology [J]. Thromb Haemost, 2001;86(1): 298-307.

共引文献1

医学研究杂志
2009年 第10期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部