纳米银诱导细胞凋亡的研究与进展

Research progress of cellular apoptosis induced by silver nanoparticles

目的:总结国内外纳米银诱导细胞凋亡的研究进展。资料来源:应用计算机检索PubMed数据库(http://www.ncbi.nlm.nih.gov/PubMed,英文)1972-08/2009-09的相关文章,检索词"silvernanoparticles,apoptosis,silver nanoparticles toxicity",限定文章语言种类为English。同时计算机检索中国期刊全文数据中国知网(www.cnki.net/index.htm,中文)1972-08/2009-09的相关文章,检索词"纳米银,凋亡,纳米银毒性",限定文章语言种类为中文。资料选择:纳入具有原创性的研究论文,其研究材料或方法具有代表性。排除重复性研究及与课题相关性较弱的文献。结局评价指标:纳米银的毒性作用及纳米银诱导细胞凋亡的机制研究。结果:目前较关注纳米银的临床运用,但是,有一些报道证实纳米银对细胞产生毒性,使细胞发生凋亡。纳米银颗粒可以通过多种途径引起细胞凋亡,而具体是哪种途径为主,则受到纳米银颗粒的粒径、粒径分布、形状、表面性质等众多因素的影响。结论:目前人们还不了解纳米银诱导细胞凋亡的机制,对纳米银颗粒引起细胞凋亡机制的研究,有利于人们改进纳米银颗粒的制造工艺或表面结构,从而减小其对细胞的毒性。

OBJECTIVE： To summarize the research progress of cellular apoptosis induced by silver nanoparticles in domestic and overseas. DATA SOURCES： The relevant articles between August 1972 and September 2009 were retrieved from PubMed （http：//www.ncbi.nlm.nih.gov/PubMed） by using the key words of “silver nanoparticles, apoptosis, silver nanoparticles toxicity and restricting the language as English by computer. Meanwhile, the relevant articles were searched from database of Chinese knowledge （CNKI www.cnki.net/index.htm） by imputing the key words of “silver nanoparticles, apoptosis, silver nanoparticles toxicity” in Chinese. DATA EXTRACTION： Hundreds of papers about silver nanoparticles were collected, 68 were selected according to the inclusive criteria. Finally, and 24 articles were analyzed. MAIN OUTCOME MEASURES： The studies regarding toxic action of silver nanoparticles, as well as the mechanisms of silver nanoparticles on cellular apoptosis. RESULTS： At present, the study of silver nanoparticles was focused on clinical application, only few reports had proved that silver nanoparticles had toxigenicity to cells, which could induce cellular apoptosis. Diverse pathways involved in cellular apoptosis induced by silver nanoparticles, however, the exactly pathway depended on particle diameter, size distribution, shape or surface property of silver nanoparticles. CONCLUSION： The mechanisms of apoptosis caused by silver nanoparticles is still poorly understood, which needs further explore. The research of apoptosis mechanisms is beneficial to updating the manufacturing process or surface structure of silver nanoparticles, therefore, reduce its cytotoxicity.