白癜风患者维生素D受体Apa I,Bsm I,Fok I基因多态性分析

Vitamin D Receptor Gene Apa I,BsmI,Fok I Polymorphism in Patients of Vitiligo

目的探讨维生素D受体Apa I,BsmI,Fok I基因多态性与白癜风的相关性。方法采用聚合酶链反应和限制性片段长度多态性方法,对46例白癜风患者和50例健康人的维生素D受体Apa I,Bsm I,Fok I基因型进行分析。结果VDR-ApaI基因AA,Aa,aa基因型分布频率在白癜风组分别为6.52%,21.74%,71.74%,对照组分别为12.00%,42.00%,46.00%;VDR-Bsm I基因BB,Bb,bb基因型分布频率在白癜风组分别为2.17%,47.83%,50.00%,对照组分别为2.00%,20.00%,78.00%;VDR-Fok I基因FF,Ff,ff基因型分布频率在白癜风组分别为32.60%,52.17%,15.22%,对照组分别为32.00%,52.00%,16.00%。维生素D受体Apa I,BsmI位点上aa,Bb基因型在白癜风患者中频率较高(P=0.036;P=0.013)。结论携带维生素D受体aa,Bb基因型可能会增加患白癜风的易感性。

Objective To investigate the association between vitamin D receptor（VDR） gene ApaⅠ,BsmⅠ,FokⅠ polymorphism and vitiligo. Methods Genotypes of VDR in 46 vitiligo patients and 50 healthy controls were tested by PCR- RFLP. Results The fi＇equency of AA in vitiligo patients was 6.52% and that was 12.00% in control. The frequency of Aa in vitiligo patients was 21.74% and that was 42.00% in control. The frequency of aa in vitiligo patients was 71.74% and that was 46.00 % in control. The frequency of BB in vitiligo patients was 2.17% and that was 2.00% in control. The frequency of Bb in vitiligo patients was 47.83% and that was 20.00% in control. The frequency of bb in vitiligo patients was 50.00% and that was 78.00% in control. The frequency of FF in vitiligo patients was 32.60% and that was 32.00% in control. The frequency of Ff in vitiligo patients was 52.17% and that was 52.00% in control. The frequency of ff in vitiligo patients was 15.22% and that was 16.00% in control. The aa,Bb genotype showed a higher frequency in vitiligo patients than that in control（P = 0. 036 ;P =0. 013）. Conclusion The aa,Bb genotype may increase the susceptibility of vitiligo.