急性脊髓损伤大鼠血糖和胰岛素浓度的变化

Changes of blood glucose and insulin following acute spinal cord injury in rats

目的探讨急性脊髓损伤大鼠血糖与血清胰岛素浓度的变化。方法192只雄性大鼠按随机对照法分为假手术组、轻度损伤组和重度损伤组。Allen法制作脊髓损伤模型。One-Touch Ultra^TM微型血糖仪测定血糖浓度，放射免疫法测定血清胰岛素浓度。在伤前及伤后1h、4h．8h、12h、1d、3d、5d，测定各组大鼠的血糖和血清胰岛素值，并计算血清胰岛素敏感指数（ISI）。结果急性脊髓损伤大鼠血糖和血清胰岛素浓度显著升高。脊髓损伤越重，血糖和血清胰岛素浓度升高越明显。轻度和重度脊髓损伤后大鼠的血清ISI均有下降，但重度损伤组大鼠血清ISI的下降更明显，且随脊髓损伤程度的增高而降低。结论急性脊髓损伤后血糖浓度的升高可能与机体存在胰岛素不敏感有关。

Objective To study the changes of blood glucose and insulin following acute spinal cord injury in rats. Methods A total of 192 male rats were randomly divided into sham group, mild injury group and severe injury group. Animal model with spinal cord injury was made by Allen＇s method. Blood glucose levels were measured by OneTouch Ultra^TM glucose measurer and blood insulin value by radioimmunoassay at pre-injury and at 1,4, 8, 12 hours, 1,3 and 5 days post-injury. Later, the insulin sensitivity index （ISI） was calculated. Results The blood glucose levels increased markedly after spinal cord injury. The severer trauma resulted in faster increase of the blood glucose and insulin levels. ISI is decreased in mild and severe injury groups, with more significant in severe injury group. ISI decreased with higher severity of spinal cord injury. Conclusion The hyperglycemia after spinal cord injury is probably associated with insulin resistance.