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完全弗氏佐剂诱导前列腺炎模型大鼠尿道腺分泌异常的研究 被引量:1

Secretory alterations of urethral glands in complete Freund's adjuvant-induced prostatitis in rats
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摘要 目的观察前列腺炎对大鼠尿道腺分泌的影响。方法20只Sprague-Dawley大鼠随机分为前列腺炎组和对照组,前列腺炎组侧叶内给予注射完全弗氏佐剂0.1ml,对照组给予注射生理盐水0.1ml,2周后取尿道腺组织做AB-PAS黏液染色和分泌型粘蛋白MUC5AC免疫组化染色。结果①前列腺炎组前列腺内大量淋巴细胞、单核细胞浸润,对照组阴性,前列腺炎动物模型建立成功。②AB-PAS黏液特殊染色提示:前列腺炎组大鼠尿道腺细胞的黏液面积、直径、光密度均比对照组高,且差异有统计学意义(P<0.05)。③分泌型粘蛋白MUC5AC免疫组化染色结果提示前列腺炎组粘蛋白面积、平均光密度与对照组相比差异有统计学意义(P<0.05)。结论前列腺炎可以导致尿道腺合成和分泌黏液功能增强,这可能是前列腺患者尿道分泌物增多的原因之一。 Objective To investigate the effects of prostatitis on secretory alterations of urethral glands in rats.Methods Twenty adult Sprague-Dawley rats were randomly divided into prostatitis group and control group.The prostatitis group(n=10) was injected with 0.1 ml Freund s complete adjuvant at each lateral lobe while the control group(n=10) with 0.1 ml 0.9% physiological saline.Two weeks later the rats were killed and the urethral glands were harvested for pathologic examination and immunohistochemical staining. Results (1) There was a great quantity of lymphcells and mononuelear cells infiltrating the prostate gland in prostatitis group while there was no inflammatory cells in control group. (2) AB-PAS pathologic examination results in urethral glands showed that the grume area, diameter, optical density in control group were significantly less than those of the prostatitis group (P 〈 0. 05 ). (3) MUC5 AC immunohistochemical staining results in urethral glands suggested that masculine particle area and average optical density of the urethral gland cells in control group were significantly less than that of the prostatitis group (P 〈0. 05 ). Conclusion Prostatitis may strengthen the synthesis and secretion of mucus in the urethral glands, which might be the reason why prostatitis patients have more urethral secretion.
出处 《第三军医大学学报》 CAS CSCD 北大核心 2009年第22期2266-2268,共3页 Journal of Third Military Medical University
关键词 前列腺炎 尿道腺 分泌 prostatitis urethral glands secretion
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同被引文献19

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