大承气汤动物含药血清对Cajal间质细胞三磷酸肌醇受体表达的影响

Serum from rats medicated with Dachengqitang significantly enhances the expression of inositol 1,4,5-triphosphate receptor mRNA in interstitial cells of Cajal in rat jejunum

目的:探讨大承气汤含药动物血清对大鼠胃肠Cajal间质细胞(ICC)三磷酸肌醇受体(IP3R)表达的影响.方法:制备大承气汤含药动物血清,作用于Wistar大鼠空肠ICC细胞,然后RT-PCR检测空白培养液组、含药血清高浓度组(100%含药血清DMEM-F12培养液)及低浓度组(20%含药血清DMEM-F12培养液)IP3R受体表达变化.结果:大鼠空肠ICC细胞3型IP3R均有表达,含药动物血清组较正常血清组明显增加(均P<0.05),高浓度组较低浓度组有显著差异(IP3R1/β-actin:1.012±0.129vs0.625±0.075;IP3R2/β-actin:0.813±0.098vs0.476±0.031;IP3R3/β-actin:0.924±0.113vs0.583±0.046,均P<0.05).结论:大承气汤动物含药血清可显著增强大鼠小肠ICCIP3R表达,并存在量效依赖关系.

AIM：To investigate the effect of serum from rats medicated with Dachengqitang on the expression of inositol 1,4,5-triphosphate receptor （IP3R） mRNA in interstitial cells of Cajal （ICC） in rat jejunum. METHODS：After rats were intragastrically ad-ministered Dachengqitang,they were killed to prepare medicated rat serum. The ICCs in rat jejunum were then isolated and treated with Dulbecco＇s Modified Eagle＇s Medium/Ham＇s Nutrient Mixture F-12 （DMEM-F12; normal control group）,100% medicated rat serum in DMEM-F12 （high-dose treatment group）,and 20% medicated rat serum in DMEM-F12 （lowdose treatment group）,respectively. The mRNA expression of IP3R subtypes in ICCs were determined by reverse transcription-polymerase chain reaction （RT-PCR）. RESULTS：All subtypes of IP3R were expressed in ICCs in rat jejunum. The mRNA expression levels of IP3R subtypes were signif icantly higher in the two serum treatment groups than in the normal control group （all P〈0.05）. Significant differences were also noted in the mRNA expression levels of IP3R subtypes between the high-dose and low-dose treatment groups （IP3R1/β-actin：1.012 ± 0.129 vs 0.625 ± 0.075; IP3R2/β-actin：0.813 ± 0.098 vs 0.476 ± 0.031; IP3R3/β-actin：0.924 ± 0.113 vs 0.583 ± 0.046,all P〈0.05）. CONCLUSION：Serum from rats medicated with Dachengqitang can signifi cantly increase the expression of IP3R mRNA in ICCs in rat jejunum in a dose-dependent manner.