ccnd1基因表达和其它肿瘤标志物在乳腺癌诊断中的应用

Clinical application of ccnd1 gene expression in the diagnosis of breast cancer

目的:探讨ccnd1基因表达水平在乳腺癌诊断和治疗监测中的应用。方法:采用荧光定量PCR(FQ-PCR)技术检测20例健康女性体检者、30例良性乳腺肿瘤和56例乳腺癌外周血中ccnd1基因表达量,用化学发光法检测其CA153、CA125和CEA,并进行对比分析。结果:ccnd1对乳腺癌诊断灵敏度显著高于CA153、CA125和CEA。4种指标在手术后均较术前显著下降。结论:ccnd1的基因表达水平可有效监测乳腺癌的诊断、治疗和预后。

Objective：To discuss the clinical application of ccndl gene expression in the diagnosis and therapy of breast cancer. Methods：Clinical samples of 20 health women,30 patients with benign breast disease and 56 patients with malign breast disease were detected by FQ -PCR. CA153, CA125 and CEA were detected by chemical luminous method. Results：The sensitivity of ccnd1 to diagnose breast cancer was higher than those of CA153, CA125 and CEA. Four index were declining after operation. Conclusion：Gene expression of ccndl can give objective evidence to the diagnosis, therapy and prognosis of breast cancer.