仙人掌对体外培养成纤维细胞生长增殖的影响

Effects of Opuntia on fibroblast proliferation in vitro

目的:观察仙人掌对体外培养成纤维细胞增殖的影响及其量-效关系。方法:以小鼠成纤维细胞株(NIH3T3)为研究对象,采用四氮唑盐(MTT)比色法测定仙人掌对成纤维细胞增生的影响(以OD值反映细胞活性),采用免疫细胞化学染色法检测成纤维细胞的增殖细胞核抗原(PCNA)的表达。结果:在一定浓度范围内(0.0625～0.5mg/mL),仙人掌能明显促进体外培养的成纤维细胞生长增殖,同时促进PCNA蛋白的表达,并随着仙人掌浓度的增加,成纤维细胞数量和PCNA蛋白的表达均不断增加,在0.5mg/mL浓度时达到高峰,呈现明显的剂量-效应关系(r分别为0.761,0.783;P均<0.01)。结论:在一定浓度范围内,仙人掌具有促进体外培养成纤维细胞增殖的作用,并表现为明显的量-效关系。

Objective： To explore the effects of Opuntia on fibroblast proliferation in vitro and its concentration-effect relationship. Methods： The effects of Opuntia on proliferation of fibroblast were measured with MTT colorimetric assay,and PCNA productions were examined with immunocytechemistry. Results： Opuntia could provoke fibroblast proliferation and increase PCNA protein expression in concen- tration range 0.0625～0.5 mg/mL. Furthermore,the effects exhibited a significant concentration-dependent relationship（r=0.761,0.783 respectively,P〈0.01）. Conclusion： Opuntia can provoke fibroblast prolif- eration within a certain concentration range, which exhibits a significant concentration-effect relationship.