摘要
目的研究人S100A6对人骨肉瘤细胞系MG63和U2OS中β-catenin的作用。方法分别用携带人S100A6及其siRNA基因的重组腺病毒AdS100A6和AdSiS100A6感染MG63和U2OS,分别使细胞中S100A6表达上调和下调;RT-PCR、蛋白印迹和免疫细胞化学法分别检测细胞中β-catenin mRNA和蛋白表达。结果上调骨肉瘤细胞系MG63和U2OS中S100A6后,β-catenin mRNA和蛋白表达均增加(P<0.05),蛋白的增加以胞核更明显。下调S100A6则使细胞中β-catenin mRNA及蛋白表达减少(P<0.05),蛋白的减少也以胞核更甚。结论增加Wnt信号途径活性可能是S100A6参与肿瘤发生发展的机制之一。
Objective To investigate the effects of human S100A6 on β-catenin in human osteosarcoma cell lines MG63 and U20S. Methods Cell lines MG63 and U20S were infected by recombinant adenovirnses carrying human S100A6 and its siRNA gene, AdS100A6 and AdSiS100A6 respectively, to up-regulate and down-regulate the expression of S100A6. Then RT-PCR,Western blot and immunocytochemistry were used to detect mRNA and protein （level and/or distribution） of β-catenin. Results In both cell lines, with up-regulated S100A6, expression of β-catenin mRNA and protein increased（ P 〈 0.05 ）and β-catenin protein increase was more obvious in nuclear than in cytoplasma; while down-regulating S100A6, both the mRNA and protein level of β-catenin decreased （P 〈 0. 05 ） ; [β-catenin protein decrease was more obvious in nuclear than in cytoplasma, too. Conclusion Increasing Wnt/β-catenin signaling activity may be a mechanism that S100A6 involves in tumor development.
出处
《基础医学与临床》
CSCD
北大核心
2009年第11期1144-1149,共6页
Basic and Clinical Medicine
基金
国家自然科学基金(30340039
30772548)
