氯沙坦降低心力衰竭大鼠心肌细胞凋亡

Losartan reduces cardiomyocyte apoptosis in rats with heart failure

目的观察氯沙坦对心力衰竭大鼠心肌细胞凋亡的影响。方法选8周龄雄性SD大鼠,分为对照组(C组)、心衰组(HF组)和治疗组(LS组)。采用腹腔注射阿霉素造模,LS组同时氯沙坦灌胃。透射电镜观察心肌超微结构,检测血清中CPK、CK-MB及LDH的含量。用原位脱氧核糖核酸酶末端标记法(TUNEL法)检测大鼠心肌细胞凋亡,免疫组化法检测大鼠心肌BAX、BCL-2蛋白的表达。结果HF组与C组相比,心肌细胞损伤明显,并可见凋亡小体,血清中CPK、CK-MB和LDH升高;LS组与HF组相比,心肌细胞凋亡指数下调(P<0.01),心肌细胞BCL-2表达增加,BAX表达减少。结论氯沙坦可有效抑制心力衰竭过程中发生的心肌细胞凋亡,逆转心肌损伤,改善心衰进程。

Objective To find the inpaet （s） of Losartan on myocardial apoptosis in rats with heart failure. Methods Eight-week-old male SD rats were divided into control group （group C）, heart failure （group HF） and treatment group（ group LS）. Use of doxorubicin injection model,group LS at the same time in a row to the oral losartan, myocardial ultrastructure was examined by TEM. DNA-situ terminal labeling （TUNEL method） was used to detect myocardial apoptosis and myocardial BAX, BCL-2 protein expression was detected by immunohistochemistry. Results Group HF showed significant myocardial cell injury, and apoptotic bodies were found, serum CPK, CK-MB and LDH were increased. In LS group, myocardial apoptosis index was lower （P 〈0. 01 ） , myocardial cells BCL-2 expression, BAX expression reduced as compared with those in HF group. Conclusion Losartan can effectively inhibit the process of heart failure occurred in cardiomyocyte apoptosis, reverse myocardial damage and improve the prognosis of heart failure.